ArticleNucleic acids research1998
Nucleosomes are not necessary for promoter-proximal pausing in vitro on the Drosophila hsp70 promoter.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
- BRCA1-BARD1 regulates transcription through modulating topoisomerase IIβ.Open biology · 2021Article
- Regulation of Promoter Proximal Pausing of RNA Polymerase II in Metazoans.Journal of molecular biology · 2021Review
- TFIID Enables RNA Polymerase II Promoter-Proximal Pausing.Molecular cell · 2020Article
- Article
- A positioned +1 nucleosome enhances promoter-proximal pausing.Nucleic acids research · 2015Article
- Distinct mechanisms of transcriptional pausing orchestrated by GAGA factor and M1BP, a novel transcription factor.The EMBO journal · 2013Article
- Kinetic competition between elongation rate and binding of NELF controls promoter-proximal pausing.Molecular cell · 2013Article
- Coupling polymerase pausing and chromatin landscapes for precise regulation of transcription.Biochimica et biophysica acta · 2012Review
- Inducible hsp70 in the regulation of cancer cell survival: analysis of chaperone induction, expression and activity.Cancers · 2011Article
- Pol II waiting in the starting gates: Regulating the transition from transcription initiation into productive elongation.Biochimica et biophysica acta · 2011Review
- Synergistic action of RNA polymerases in overcoming the nucleosomal barrier.Nature structural & molecular biology · 2010Article
- Promoter proximal pausing on genes in metazoans.Chromosoma · 2009Review
- Molecular characterization of Drosophila NELF.Nucleic acids research · 2005Article
- NELF and DSIF cause promoter proximal pausing on the hsp70 promoter in Drosophila.Genes & development · 2003Article
- Localized recruitment of a chromatin-remodeling activity by an activator in vivo drives transcriptional elongation.Genes & development · 2003Article
- Potential targets for HSF1 within the preinitiation complex.Cell stress & chaperones · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
RNA polymerase II has been found to pause stably on several metazoan genes in a promoter-proximal region located 20-40 nt downstream from the start site of transcription. Escape of polymerase from this paused state has been proposed to be a rate limiting step in transcription of some genes. A study of the human hsp70 promoter showed that a nucleosome positioned downstream from the transcription start was a key component in establishing a stably paused polymerase in one cell-free system. We tested whether these results could be extended to the Drosophila hsp70 promoter in a Drosophila cell-free system and found that polymerase paused stably on the promoter even when the length of DNA downstream from the transcription start was not sufficient for assembly of a nucleosome. Our results indicate that a downstream nucleosome is not a universal requirement for stably pausing RNA polymerase in the promoter-proximal region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.