Transcription initiation from a poly(dA) tract.

D H Shain, M X Zuber, T Neuman

Open access · bronzeAbstract read

In one paragraph

Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.5field-weighted citation impact, top 37% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

D H ShainDepartment of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO 80523, USA. dshain@mendel.berkeley.edu

M X Zuber

T Neuman

Colorado State University · US

Funding

ME1 DURING NEURONAL DIFFERENTIATIONR01NS033804 · NINDS · COLORADO STATE UNIVERSITY-FORT COLLINS · PI BAMBURG, JAMES R · 1994 to 1996

–

NINDS NIH HHS NS33804

6 · The paper itself

Abstract

The mammalian ME1 gene encodes a non-tissue-specific, helix-loop-helix transcription factor that is enriched in morphogenetically active regions during development. Regulation of mouse ME1 gene expression is controlled by a novel initiator (ME1 Inr) that promotes transcription from the center of a 13 bp poly(dA) tract. We show here that the ME1 Inr autonomously directs initiation from the poly(dA) tract both in vitro and in vivo. This transcription was dependent upon two protein complexes; MBPalpha, which associated directly with the poly(dA) tract, and MBPbeta, which introduced an approximately 60 degrees bend immediately downstream of the poly(dA) tract. The MBPalpha and MBPbeta binding sites were strikingly conserved in homologous DNA from several mammalian species and the frog Xenopus laevis. These results suggest that the ME1 Inr constitutes a robust nucleation site that promotes transcription initiation in the absence of conventional promoter elements.

Indexed as

Transcription, GeneticXenopus Proteins3T3 CellsAnimalsBase SequenceBasic Helix-Loop-Helix ProteinsBinding SitesCell LineDNADNA-Binding ProteinsEvolution, MolecularGene Expression Regulation, DevelopmentalHeLa CellsHelix-Loop-Helix MotifsHumansIn Vitro TechniquesBasic Helix-Loop-Helix ProteinsDNADNA-Binding ProteinsPoly Apoly(dA)TCF12 protein, humanTcf12 protein, mouseTCF12 protein, XenopusTranscription FactorsXenopus Proteins

Identifiers

PMID9461462

DOI10.1093/nar/26.4.1019

PMCPMC147351

OpenAlexW2072269012

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.