ArticleNucleic acids research1998
Translational termination in Escherichia coli: three bases following the stop codon crosslink to release factor 2 and affect the decoding efficiency of UGA-containing signals.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Article
- The endoribonuclease Rae1 from Bacillus subtilis cleaves mRNA upstream of stalled ribosomes.Nucleic acids research · 2025Article
- Differential Selection for Translation Efficiency Shapes Translation Machineries in Bacterial Species.Microorganisms · 2024Article
- Analysis of programmed frameshifting during translation ofRNA (New York, N.Y.) · 2024Article
- Article
- Article
- In eubacteria, unlike eukaryotes, there is no evidence for selection favouring fail-safe 3' additional stop codons.PLoS genetics · 2019Article
- Purifying and positive selection in the evolution of stop codons.Scientific reports · 2018Article
- Article
- Evolutionary Dynamics of Abundant Stop Codon Readthrough.Molecular biology and evolution · 2016Article
- Nonsense codon suppression in fission yeast due to mutations of tRNA(Ser.11) and translation release factor Sup35 (eRF3).Current genetics · 2015Article
- The highly conserved codon following the slippery sequence supports -1 frameshift efficiency at the HIV-1 frameshift site.PloS one · 2015Article
- Enhanced yield of recombinant proteins with site-specifically incorporated unnatural amino acids using a cell-free expression system.PloS one · 2013Article
- Evidence of abundant stop codon readthrough in Drosophila and other metazoa.Genome research · 2011Article
- Mutation in the TCRα subunit constant gene (TRAC) leads to a human immunodeficiency disorder characterized by a lack of TCRαβ+ T cells.The Journal of clinical investigation · 2011Article
- Review
- Crystal structure of a translation termination complex formed with release factor RF2.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Comparison of characteristics and function of translation termination signals between and within prokaryotic and eukaryotic organisms.Nucleic acids research · 2006Article
- Leaky termination at premature stop codons antagonizes nonsense-mediated mRNA decay in S. cerevisiae.RNA (New York, N.Y.) · 2004Article
- Comparative study of translation termination sites and release factors (RF1 and RF2) in procaryotes.Journal of molecular evolution · 2003Article
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4 authors.
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Abstract
The observations that the Escherichia coli release factor 2 (RF2) crosslinks with the base following the stop codon (+4 N), and that the identity of this base strongly influences the decoding efficiency of stop signals, stimulated us to determine whether there was a more extended termination signal for RF2 recognition. Analysis of the 3' contexts of the 1248 genes in the E.coli genome terminating with UGA showed a strong bias for U in the +4 position and a general bias for A and against C in most positions to +10, consistent with the concept of an extended sequence element. Site-directed crosslinking occurred to RF2 from a thio-U sited at the +4, +5 and +6 bases following the UGA stop codon but not beyond (+7 to +10). Varying the +4 to +6 bases modulated the strength of the crosslink from the +1 invariant U to RF2. A strong selection bias for particular bases in the +4 to +6 positions of certain E. coli UGANNN termination sites correlated in some cases with crosslinking efficiency to RF2 and in vivo termination signal strength. These data suggest that RF2 may recognise at least a hexanucleotide UGA-containing sequence and that particular base combinations within this sequence influence termination signal decoding efficiency.
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