ArticleJournal of bacteriology1998
Immunolocalization of Hsp60 in Legionella pneumophila.
Article in Journal of bacteriology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 113 citations in OpenAlex.
- The Passage of Chaperonins to Extracellular Locations inBiomolecules · 2025Article
- The Functional Differences between the GroEL Chaperonin ofBiomolecules · 2021Article
- Secretion Systems in Gram-Negative Bacterial Fish Pathogens.Frontiers in microbiology · 2021Review
- Comparative Genomic Analysis of Two Chilean Renibacterium salmoninarum Isolates and the Type Strain ATCC 33209T.Genome biology and evolution · 2018Article
- Identification of Cross Reactive Antigens of C. botulinum Types A, B, E & F by Immunoproteomic Approach.Current microbiology · 2018Article
- Intestinal M cells.Journal of biochemistry · 2016Review
- Transcriptome analysis of cyst formation in Rhodospirillum centenum reveals large global changes in expression during cyst development.BMC genomics · 2015Article
- Abundance of the multiheme c-type cytochrome OmcB increases in outer biofilm layers of electrode-grown Geobacter sulfurreducens.PloS one · 2014Article
- Legionella pneumophila requires polyamines for optimal intracellular growth.Journal of bacteriology · 2011Article
- DsbA2 (27 kDa Com1-like protein) of Legionella pneumophila catalyses extracytoplasmic disulphide-bond formation in proteins including the Dot/Icm type IV secretion system.Molecular microbiology · 2011Article
- The Legionella pneumophila Chaperonin - An Unusual Multifunctional Protein in Unusual Locations.Frontiers in microbiology · 2011Article
- The purified and recombinant Legionella pneumophila chaperonin alters mitochondrial trafficking and microfilament organization.Infection and immunity · 2009Article
- Outer membrane vesicle-mediated release of cytolethal distending toxin (CDT) from Campylobacter jejuni.BMC microbiology · 2009Article
- Mycobacterium tuberculosis Cpn60.2 and DnaK are located on the bacterial surface, where Cpn60.2 facilitates efficient bacterial association with macrophages.Infection and immunity · 2009Article
- Proinflammatory effect in whole blood by free soluble bacterial components released from planktonic and biofilm cells.BMC microbiology · 2008Article
- Proteomic characterization of the whole secretome of Legionella pneumophila and functional analysis of outer membrane vesicles.Infection and immunity · 2008Article
- Packaging of live Legionella pneumophila into pellets expelled by Tetrahymena spp. does not require bacterial replication and depends on a Dot/Icm-mediated survival mechanism.Applied and environmental microbiology · 2008Article
- Surface-exposed proteins of Ehrlichia chaffeensis.Infection and immunity · 2007Article
- Identification, recombinant expression, immunolocalization in macrophages, and T-cell responsiveness of the major extracellular proteins of Francisella tularensis.Infection and immunity · 2006Article
- Stress wars: the direct role of host and bacterial molecular chaperones in bacterial infection.Infection and immunity · 2006Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the most abundant proteins synthesized by Legionella pneumophila, particularly during growth in a variety of eukaryotic host cells, is Hsp60, a member of the GroEL family of molecular chaperones. The present study was initiated in response to a growing number of reports suggesting that for some bacteria, including L. pneumophila, Hsp60 may exist in extracytoplasmic locations. Immunolocalization techniques with Hsp60-specific monoclonal and polyclonal antibodies were used to define the subcellular location and distribution of Hsp60 in L. pneumophila grown in vitro, or in vivo inside of HeLa cells. For comparative purposes Escherichia coli, expressing recombinant L. pneumophila Hsp60, was employed. In contrast to E. coli, where Hsp60 was localized exclusively in the cytoplasm, in L. pneumophila Hsp60 was predominantly associated with the cell envelope, conforming to a distribution pattern typical of surface molecules that included the major outer membrane protein OmpS and lipopolysaccharide. Interestingly, heat-shocked L. pneumophila organisms exhibited decreased overall levels of cell-associated Hsp60 epitopes and increased relative levels of surface epitopes, suggesting that Hsp60 was released by stressed bacteria. Putative secretion of Hsp60 by L. pneumophila was further indicated by the accumulation of Hsp60 in the endosomal space, between replicating intracellular bacteria. These results are consistent with an extracytoplasmic location for Hsp60 in L. pneumophila and further suggest both the existence of a novel secretion mechanism (not present in E. coli) and a potential role in pathogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.