ArticleMammalian genome : official journal of the International Mammalian Genome Society1998
Construction and extensive characterization of a goat bacterial artificial chromosome library with threefold genome coverage.
Article in Mammalian genome : official journal of the International Mammalian Genome Society, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 60 citations in OpenAlex.
- Candidate Genes and Their Expressions Involved in the Regulation of Milk and Meat Production and Quality in Goats (Animals : an open access journal from MDPI · 2022Review
- Article
- Construction and analysis of Siberian tiger bacterial artificial chromosome library with approximately 6.5-fold genome equivalent coverage.International journal of molecular sciences · 2014Article
- Design and characterization of a 52K SNP chip for goats.PloS one · 2014Article
- Construction and preliminary characterization analysis of Wuzhishan miniature pig bacterial artificial chromosome library with approximately 8-fold genome equivalent coverage.BioMed research international · 2013Article
- Goat RSPO1 over-expression rescues sex-reversal in Rspo1-knockout XX mice but does not perturb testis differentiation in XY or sex-reversed XX mice.Transgenic research · 2009Article
- Complete genomic sequence of the goat prion protein gene (PRNP).Virus genes · 2009Article
- An extended river buffalo (Bubalus bubalis, 2n = 50) cytogenetic map: assignment of 68 autosomal loci by FISH-mapping and R-banding and comparison with human chromosomes.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Article
- An advanced sheep (Ovis aries, 2n = 54) cytogenetic map and assignment of 88 new autosomal loci by fluorescence in situ hybridization and R-banding.Animal genetics · 2007Article
- Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.BMC biotechnology · 2005Article
- Identification by R-banding and FISH of chromosome arms involved in Robertsonian translocations in several deer species.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2003Article
- Conditionally amplifiable BACs: switching from single-copy to high-copy vectors and genomic clones.Genome research · 2002Article
- Markedly increased susceptibility to natural sheep scrapie of transgenic mice expressing ovine prp.Journal of virology · 2001Article
- Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- How malleable is the eukaryotic genome? Extreme rate of chromosomal rearrangement in the genus Drosophila.Genome research · 2001Article
- Fine mapping suggests that the goat Polled Intersex Syndrome and the human Blepharophimosis Ptosis Epicanthus Syndrome map to a 100-kb homologous region.Genome research · 2000Article
- A high-density physical map of Sinorhizobium meliloti 1021 chromosome derived from bacterial artificial chromosome library.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Position-independent and copy-number-related expression of a goat bacterial artificial chromosome alpha-lactalbumin gene in transgenic mice.The Biochemical journal · 1999Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A goat Bacterial Artificial Chromosome (BAC) library of 61,440 independent clones was constructed and characterized. The average size of the inserts was estimated at 153 kilobases by analyzing almost 500 clones using Not1 digestion followed by FIGE (Field Inverted Gel Electrophoresis) analysis. The library represents about three genome equivalents, which yields a theoretical probability of 0.95 of isolating a particular DNA sequence. After individual growth, the clones were arrayed in 40 superpools, which were organized in three dimension pools. A rapid technique for pool DNA preparation by microwave treatment was set up. This technique was compatible with PCR analysis. Primer pairs from 166 sequences (microsatellites, coding sequences from goat, and conserved Expressed Sequence Tags (ESTs) from humans) enabled the library to be successfully searched in 165 cases, with an average of 3.52 positive superpools. Only one sequence could not be found. The degree of chimerism was evaluated by FISH analysis with DNA from over 110 clones and was estimated at 4%. This BAC library will constitute an invaluable tool for positional cloning in ruminants, as well as for more general comparative mapping studies in mammals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.