ArticleThe Journal of physiology1997
Inhibition of ATP-activated current by zinc in dorsal root ganglion neurones of bullfrog.
Article in The Journal of physiology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Physiological Concentrations of Zinc Have Dual Effects on P2X Myenteric Receptors of Guinea Pig.Cellular and molecular neurobiology · 2018Article
- Mitochondrial dysfunctions in myalgic encephalomyelitis/chronic fatigue syndrome explained by activated immuno-inflammatory, oxidative and nitrosative stress pathways.Metabolic brain disease · 2014Article
- Potent and long-lasting inhibition of human P2X2 receptors by copper.Neuropharmacology · 2014Article
- Activation and regulation of purinergic P2X receptor channels.Pharmacological reviews · 2011Review
- Ethanol is a fast channel inhibitor of P2X4 receptors.The Journal of pharmacology and experimental therapeutics · 2011Article
- Amyloid-beta induces a caspase-mediated cleavage of P2X4 to promote purinotoxicity.Neuromolecular medicine · 2009Article
- Opposite effects of zinc on human and rat P2X2 receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- The mechanism by which ethanol inhibits rat P2X4 receptors is altered by mutation of histidine 241.British journal of pharmacology · 2005Article
- Modulation of ATP-induced currents by zinc in acutely isolated hypothalamic neurons of the rat.British journal of pharmacology · 2003Article
- Fast synaptic transmission mediated by P2X receptors in CA3 pyramidal cells of rat hippocampal slice cultures.The Journal of physiology · 2001Article
- Multiple P2X receptors on guinea-pig pelvic ganglion neurons exhibit novel pharmacological properties.British journal of pharmacology · 2001Article
- Inhibition by ethanol of rat P2X(4) receptors expressed in Xenopus oocytes.British journal of pharmacology · 2000Article
- Rat chromaffin cells lack P2X receptors while those of the guinea-pig express a P2X receptor with novel pharmacology.British journal of pharmacology · 1999Article
- The effects of inflammation and inflammatory mediators on nociceptive behaviour induced by ATP analogues in the rat.British journal of pharmacology · 1999Article
- Effects of Zn2+ on wild and mutant neuronal alpha7 nicotinic receptors.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The antagonist trinitrophenyl-ATP reveals co-existence of distinct P2X receptor channels in rat nodose neurones.The Journal of physiology · 1998Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
1. The effect of Zn2+ on ATP-activated current was studied in bullfrog dorsal root ganglion (DRG) neurones using the whole-cell patch-clamp technique. 2. Zn2+ (2-800 microM) inhibited current activated by submaximal concentrations of ATP. The Zn2+ concentration that produced 50% inhibition (IC50) of current activated by 2.5 microM ATP was 61 +/- 9.8 microM. When ATP concentrations were adjusted to account for chelation of Zn2+, the IC50 of Zn2+ was 86 +/- 18 microM. 3. The inhibitory action of Zn2+ on ATP-gated channels did not appear to be due to a decrease in the concentration of one or more species of ATP. 4. Zn2+ inhibition of ATP-activated current was independent of membrane potential between -80 and +40 mV, and did not involve a shift in the reversal potential of the current. 5. Zn2+ (100 microM) shifted the ATP concentration-response curve to the right in a parallel manner, increasing the EC50 for ATP from 2.5 +/- 0.5 microM to 5.5 +/- 0.4 microM. 6. Zn2+ decreased the time constant of deactivation of ATP-gated ion channels without affecting the time constant of activation or desensitization. 7. Dithiothreitol (DTT) reversed Zn2+ inhibition of ATP-activated current. 8. 2-Methylthio ATP, alpha,beta-methylene ATP and ADP activated current with EC50 values of 2.4 +/- 0.3. 50.1 +/- 5.8 and 303.1 +/- 53.9 microM, respectively. Adenosine, AMP or beta,gamma-methylene ATP did not evoke detectable current. 9. Reactive Blue 2 and pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid inhibited ATP-activated current. 10. The results suggest that Zn2+ can inhibit P2X purinoceptor function by decreasing the affinity of the binding site for ATP. These observations provide the first evidence for this action of Zn2+ on a neurotransmitter-gated ion channel. Furthermore, the receptor-channel in these neurones appears to be a novel member of the P2X purinoceptor class.
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