ArticleThe Journal of cell biology1998
Caveolin transfection results in caveolae formation but not apical sorting of glycosylphosphatidylinositol (GPI)-anchored proteins in epithelial cells.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 138 citations in OpenAlex.
- Glycosphingolipid and Glycosylphosphatidylinositol Affect Each Other in and on the Cell.Chembiochem : a European journal of chemical biology · 2023Review
- Reduced caveolae density in arteries of SHR contributes to endothelial dysfunction and ROS production.Scientific reports · 2019Article
- Prion strains depend on different endocytic routes for productive infection.Scientific reports · 2017Article
- Nerve Growth Factor Signaling from Membrane Microdomains to the Nucleus: Differential Regulation by Caveolins.International journal of molecular sciences · 2017Article
- Caveolin-1 mediated uptake via langerin restricts HIV-1 infection in human Langerhans cells.Retrovirology · 2014Article
- Discrepancy between fluorescence correlation spectroscopy and fluorescence recovery after photobleaching diffusion measurements of G-protein-coupled receptors.Analytical biochemistry · 2013Article
- Targeting receptor-mediated endocytotic pathways with nanoparticles: rationale and advances.Advanced drug delivery reviews · 2013Review
- A role for G-proteins in directing G-protein-coupled receptor-caveolae localization.Biochemistry · 2012Article
- Interaction between nitric oxide signaling and gap junctions: effects on vascular function.Biochimica et biophysica acta · 2012Review
- Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.Physiological reviews · 2012Review
- Mitochondrial cholesterol: a connection between caveolin, metabolism, and disease.Traffic (Copenhagen, Denmark) · 2011Article
- Transcriptional repression of Caveolin-1 (CAV1) gene expression by GATA-6 in bladder smooth muscle hypertrophy in mice and human beings.The American journal of pathology · 2011Article
- Modulation of Ca²+ activity in cardiomyocytes through caveolae-Gαq interactions.Biophysical journal · 2011Article
- The effect of membrane domains on the G protein-phospholipase Cbeta signaling pathway.Critical reviews in biochemistry and molecular biology · 2010Review
- Cystic fibrosis transmembrane conductance regulator and caveolin-1 regulate epithelial cell internalization of Pseudomonas aeruginosa.American journal of physiology. Cell physiology · 2009Article
- Endocytosis via caveolae: alternative pathway with distinct cellular compartments to avoid lysosomal degradation?Journal of cellular and molecular medicine · 2009Review
- Lipid rafts and clathrin cooperate in the internalization of PrP in epithelial FRT cells.PloS one · 2009Article
- The activity of the epithelial sodium channels is regulated by caveolin-1 via a Nedd4-2-dependent mechanism.The Journal of biological chemistry · 2009Article
- Caveolin-2 is required for apical lipid trafficking and suppresses basolateral recycling defects in the intestine of Caenorhabditis elegans.Molecular biology of the cell · 2009Article
- TGFbeta-induced RhoA activation and fibronectin production in mesangial cells require caveolae.American journal of physiology. Renal physiology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Most epithelial cells sort glycosylphosphatidylinositol (GPI)-anchored proteins to the apical surface. The "raft" hypothesis, based on data mainly obtained in the prototype cell line MDCK, postulates that apical sorting depends on the incorporation of apical proteins into cholesterol/glycosphingolipid (GSL) rafts, rich in the cholesterol binding protein caveolin/VIP21, in the Golgi apparatus. Fischer rat thyroid (FRT) cells constitute an ideal model to test this hypothesis, since they missort both endogenous and transfected GPI-anchored proteins to the basolateral plasma membrane and fail to incorporate them into cholesterol/glycosphingolipid clusters. Because FRT cells lack caveolin, a major component of the caveolar coat that has been proposed to have a role in apical sorting of GPI-anchored proteins (Zurzolo, C., W. Van't Hoff, G. van Meer, and E. Rodriguez-Boulan. 1994. EMBO [Eur. Mol. Biol. Organ.] J. 13:42-53.), we carried out experiments to determine whether the lack of caveolin accounted for the sorting/clustering defect of GPI-anchored proteins. We report here that FRT cells lack morphological caveolae, but, upon stable transfection of the caveolin1 gene (cav1), form typical flask-shaped caveolae. However, cav1 expression did not redistribute GPI-anchored proteins to the apical surface, nor promote their inclusion into cholesterol/GSL rafts. Our results demonstrate that the absence of caveolin1 and morphologically identifiable caveolae cannot explain the inability of FRT cells to sort GPI-anchored proteins to the apical domain. Thus, FRT cells may lack additional factors required for apical sorting or for the clustering with GSLs of GPI-anchored proteins, or express factors that inhibit these events. Alternatively, cav1 and caveolae may not be directly involved in these processes.
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