ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Acetylcholinesterase enhances neurite growth and synapse development through alternative contributions of its hydrolytic capacity, core protein, and variable C termini.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 199 citations in OpenAlex.
- Indomethacin restores loss of hippocampal neurogenesis and cholinergic innervation and reduces innate immune expression and reversal learning deficits in adult male and female rats following adolescent ethanol exposure.Alcohol, clinical & experimental research · 2023Article
- Altered levels of variant cholinesterase transcripts contribute to the imbalanced cholinergic signaling in Alzheimer's and Parkinson's disease.Frontiers in molecular neuroscience · 2022Article
- Article
- Erythrocyte acetylcholinesterase as biomarker of pesticide exposure: new and forgotten insights.Environmental science and pollution research international · 2018Review
- Characterization of the Fifth Putative Acetylcholinesterase in the Wolf Spider, Pardosa pseudoannulata.Molecules (Basel, Switzerland) · 2017Article
- Increased Expression of Readthrough Acetylcholinesterase Variants in the Brains of Alzheimer's Disease Patients.Journal of Alzheimer's disease : JAD · 2016Article
- Effects of 4-methylbenzylidene camphor (4-MBC) on neuronal and muscular development in zebrafish (Danio rerio) embryos.Environmental science and pollution research international · 2016Article
- Primary Investigation for the Mechanism of Biatractylolide from Atractylodis Macrocephalae Rhizoma as an Acetylcholinesterase Inhibitor.Evidence-based complementary and alternative medicine : eCAM · 2016Article
- Three N-Glycosylation Sites of Human Acetylcholinesterase Shares Similar Glycan Composition.Journal of molecular neuroscience : MN · 2015Article
- A highly stable minimally processed plant-derived recombinant acetylcholinesterase for nerve agent detection in adverse conditions.Scientific reports · 2015Article
- Characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish.Fish physiology and biochemistry · 2014Article
- Acetylcholinesterase protein level is preserved in the Alzheimer's brain.Journal of molecular neuroscience : MN · 2014Article
- Interaction of acetylcholinesterase with neurexin-1β regulates glutamatergic synaptic stability in hippocampal neurons.Molecular brain · 2014Article
- Acetylcholinesterase as a biomarker in environmental and occupational medicine: new insights and future perspectives.BioMed research international · 2013Review
- Mouse acetylcholinesterase enhances neurite outgrowth of rat R28 cells through interaction with laminin-1.PloS one · 2012Article
- Trans-acting factors governing acetylcholinesterase mRNA metabolism in neurons.Frontiers in molecular neuroscience · 2012Article
- Chlorpyrifos-oxon disrupts zebrafish axonal growth and motor behavior.Toxicological sciences : an official journal of the Society of Toxicology · 2011Article
- Dose-related gene expression changes in forebrain following acute, low-level chlorpyrifos exposure in neonatal rats.Toxicology and applied pharmacology · 2010Article
- Targeting acetylcholinesterase to membrane rafts: a function mediated by the proline-rich membrane anchor (PRiMA) in neurons.The Journal of biological chemistry · 2010Article
- Upregulation of alpha7 Nicotinic Receptors by Acetylcholinesterase C-Terminal Peptides.PloS one · 2009Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Accumulated indirect evidence suggests nerve growth-promoting activities for acetylcholinesterase (AChE). To determine unequivocally whether such activities exist, whether they are related to the capacities of this enzyme to hydrolyze acetylcholine and enhance synapse development, and whether they are associated with alternative splicing variants of AChEmRNA, we used four recombinant human AChEDNA vectors. When Xenopus laevis embryos were injected with a vector expressing the synapse-characteristic human AChE-E6, which contains the exon 6-encoded C terminus, cultured spinal neurons expressing this enzyme grew threefold faster than co-cultured control neurons. Similar enhancement occurred in neurons expressing an insertion-inactivated human AChE-E6-IN protein, containing the same C terminus, and displaying indistinguishable immunochemical and electrophoretic migration properties from AChE-E6, but incapable of hydrolyzing acetylcholine. In contrast, the nonsynaptic secretory human AChE-I4, which contains the pseudointron 4-derived C terminus, did not affect neurite growth. Moreover, no growth promotion occurred in neurons expressing the catalytically active C-terminally truncated human AChE-E4, demonstrating a dominant role for the E6-derived C terminus in neurite extension. Also, AChE-E6 was the only active enzyme variant to be associated with Xenopus membranes. However, postsynaptic length measurements demonstrated that both AChE-E6 and AChE-E4 enhanced the development of neuromuscular junctions in vivo, unlike the catalytically inert AChE-E6-IN and the nonsynaptic AChE-I4. These findings demonstrate an evolutionarily conserved synaptogenic activity for AChE that depends on its hydrolytic capacity but not on its membrane association. Moreover, this synaptogenic effect differs from the growth-promoting activity of AChE, which is unrelated to its hydrolytic capacity yet depends on its exon 6-mediated membrane association.
Indexed as
Identifiers
9454834PMC6792736W1954300187What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.