ArticleInfection and immunity1998
Identification of a putative precursor to the major surface glycoprotein of Pneumocystis carinii.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Fungal antigenic variation using mosaicism and reassortment of subtelomeric genes' repertoires.Nature communications · 2023Article
- The Promise of Lung Organoids for Growth and Investigation ofFrontiers in fungal biology · 2021Article
- Diversity and Complexity of the Large Surface Protein Family in the Compacted Genomes of MultiplemBio · 2020Article
- Gene Expression of Pneumocystis murina after Treatment with Anidulafungin Results in Strong Signals for Sexual Reproduction, Cell Wall Integrity, and Cell Cycle Arrest, Indicating a Requirement for Ascus Formation for Proliferation.Antimicrobial agents and chemotherapy · 2018Article
- Expression of Pneumocystis jirovecii major surface glycoprotein in Saccharomyces cerevisiae.The Journal of infectious diseases · 2013Article
- Telomere components as potential therapeutic targets for treating microbial pathogen infections.Frontiers in oncology · 2012Article
- Complexity of the MSG gene family of Pneumocystis carinii.BMC genomics · 2009Article
- Genetic characterization of the UCS and Kex1 loci of Pneumocystis jirovecii.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2009Article
- Pneumocystis murina MSG gene family and the structure of the locus associated with its transcription.Fungal genetics and biology : FG & B · 2007Article
- Gene arrays at Pneumocystis carinii telomeres.Genetics · 2005Article
- A single-copy gene encodes Kex1, a serine endoprotease of Pneumocystis jiroveci.Infection and immunity · 2003Article
- Genetics of surface antigen expression in Pneumocystis carinii.Infection and immunity · 2001Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
The major surface glycoprotein (MSG) of Pneumocystis carinii f. sp. carinii is a family of proteins encoded by a family of heterogeneous genes. Messenger RNAs encoding different MSGs each begin with the same 365-bp sequence, called the Upstream Conserved Sequence (UCS), which is in frame with the contiguous MSG sequence. The UCS contains several potential start sites for translation. To determine if translation of MSG mRNAs begins in the UCS, polyclonal antiserum was raised against the 123-amino-acid peptide encoded by the UCS. The anti-UCS serum reacted with a P. carinii protein that migrated at 170 kDa; however, it did not react with the mature MSG protein, which migrates at 116 kDa. A 170-kDa protein was immunoprecipitated with anti-UCS serum and shown to react with a monoclonal antibody against a conserved MSG epitope. To explore the functional role of the UCS in the trafficking of MSG, the nucleotide sequence encoding the UCS peptide was ligated to the 5' end of an MSG gene and incorporated into a recombinant baculovirus. Insect cells infected with the UCS-MSG hybrid gene expressed a 160-kDa protein which was N-glycosylated. By contrast, insect cells infected with a baculovirus carrying an MSG gene lacking the UCS expressed a nonglycosylated 130-kDa protein. These data suggest that in P. carinii, translation begins in the UCS to produce a pre-MSG protein, which is subsequently directed to the endoplasmic reticulum and processed to the mature form by proteolytic cleavage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.