ArticleInfection and immunity1998
B-cell epitopes and quantification of the ESAT-6 protein of Mycobacterium tuberculosis.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 98 citations in OpenAlex.
- Tuberculin-purified protein derivative-, MPT-64-, and ESAT-6-stimulated gamma interferon responses in medical students before and after Mycobacterium bovis BCG vaccination and in patients with tuberculosis.Clinical and diagnostic laboratory immunology · 1999Trial
- Evaluation of the role ofMicrobiology spectrum · 2025Article
- Marine sponge microbe provides insights into evolution and virulence of the tubercle bacillus.PLoS pathogens · 2024Article
- The ESX-1 Substrate PPE68 Has a Key Function in ESX-1-Mediated Secretion in Mycobacterium marinum.mBio · 2022Article
- Review
- Identification of gene fusion events inNAR genomics and bioinformatics · 2020Article
- Modification of a PE/PPE substrate pair reroutes an Esx substrate pair from the mycobacterial ESX-1 type VII secretion system to the ESX-5 system.The Journal of biological chemistry · 2020Article
- Retention of EsxA in the Capsule-Like Layer ofInfection and immunity · 2019Article
- Unexpected Genomic and Phenotypic Diversity of Mycobacterium africanum Lineage 5 Affects Drug Resistance, Protein Secretion, and Immunogenicity.Genome biology and evolution · 2018Article
- EspH is a hypervirulence factor for Mycobacterium marinum and essential for the secretion of the ESX-1 substrates EspE and EspF.PLoS pathogens · 2018Article
- Antibody response against PhoP efficiently discriminates among healthy individuals, tuberculosis patients and their contacts.PloS one · 2017Article
- The ESX-5 System of Pathogenic Mycobacteria Is Involved In Capsule Integrity and Virulence through Its Substrate PPE10.PLoS pathogens · 2016Article
- Thermal stability of self-assembled peptide vaccine materials.Acta biomaterialia · 2016Article
- Differential in vivo expression of mycobacterial antigens in Mycobacterium tuberculosis infected lungs and lymph node tissues.BMC infectious diseases · 2014Article
- Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection.Memorias do Instituto Oswaldo Cruz · 2013Article
- A novel B-cell epitope identified within Mycobacterium tuberculosis CFP10/ESAT-6 protein.PloS one · 2013Article
- Immunogenicity of recombinant Mycobacterium bovis bacille Calmette-Guèrin clones expressing T and B cell epitopes of Mycobacterium tuberculosis antigens.BMC immunology · 2013Article
- Unexpected link between lipooligosaccharide biosynthesis and surface protein release in Mycobacterium marinum.The Journal of biological chemistry · 2012Article
- Expression and immunogenicity of the mycobacterial Ag85B/ESAT-6 antigens produced in transgenic plants by elastin-like peptide fusion strategy.Journal of biomedicine & biotechnology · 2010Article
- Proposing low-similarity peptide vaccines against Mycobacterium tuberculosis.Journal of biomedicine & biotechnology · 2010Article
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
ESAT-6 is an important T-cell antigen recognized by protective T cells in animal models of infection with Mycobacterium tuberculosis. In an enzyme-linked immunosorbent assay (ELISA) with overlapping peptides spanning the sequence of ESAT-6, monoclonal antibody HYB76-8 reacted with two peptides in the N-terminal region of the molecule. Assays with synthetic truncated peptides allowed a precise mapping of the epitope to the residues EQQWNFAGIEAAA at positions 3 to 15. Hydrophilicity plots revealed one hydrophilic area at the N terminus and two additional areas further along the polypeptide chain. Antipeptide antibodies were generated by immunization with synthetic 8-mer peptides corresponding to these two regions coupled to keyhole limpet hemocyanin. Prolonged immunization with a 23-mer peptide (positions 40 to 62) resulted in the formation of antibodies reacting with the peptide as well as native ESAT-6. A double-antibody ELISA was then developed with monoclonal antibody HYB76-8 as a capture antibody, antigen for testing in the second layer, and antipeptide antibody in the third layer. The assay was suitable for quantification of ESAT-6 in M. tuberculosis antigen preparations, showing no reactivity with M. bovis BCG Tokyo culture fluid, used as a negative control, or with MPT64 or antigen 85B, previously shown to cross-react with HYB76-8. This capture ELISA permitted the identification of ESAT-6 expression from vaccinia virus constructs containing the esat-6 gene; this expression could not be identified by standard immunoblotting.
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