ArticleInfection and immunity1998
Identification and characterization of a novel extracellular ferric reductase from Mycobacterium paratuberculosis.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- Systematic Assessment ofMicroorganisms · 2020Review
- The Role of Iron Competition in the Antagonistic Action of the Rice Endophyte Streptomyces sporocinereus OsiSh-2 Against the Pathogen Magnaporthe oryzae.Microbial ecology · 2018Article
- Iron Acquisition in Mycobacterium avium subsp. paratuberculosis.Journal of bacteriology · 2015Article
- Role of porins in iron uptake by Mycobacterium smegmatis.Journal of bacteriology · 2010Article
- Growth, Fe3+ reductase activity, and siderophore production by Paenibacillus polymyxa SQR-21 under differential iron conditions.Current microbiology · 2010Article
- Type IV secretion machinery promotes ton-independent intracellular survival of Neisseria gonorrhoeae within cervical epithelial cells.Infection and immunity · 2010Article
- Vibrio cholerae VciB promotes iron uptake via ferrous iron transporters.Journal of bacteriology · 2008Article
- The secreted pyomelanin pigment of Legionella pneumophila confers ferric reductase activity.Infection and immunity · 2007Article
- Isolation of high-affinity single-chain antibodies against Mycobacterium avium subsp. paratuberculosis surface proteins from sheep with Johne's disease.Clinical and vaccine immunology : CVI · 2006Article
- Mechanistic aspects of biosynthesis of silver nanoparticles by several Fusarium oxysporum strains.Journal of nanobiotechnology · 2005Article
- Glutathione-dependent extracellular ferric reductase activities in dimorphic zoopathogenic fungi.Microbiology (Reading, England) · 2005Article
- A 38-kilobase pathogenicity island specific for Mycobacterium avium subsp. paratuberculosis encodes cell surface proteins expressed in the host.Infection and immunity · 2004Article
- Distinct differences in repertoires of low-molecular-mass secreted antigens of Mycobacterium avium complex and Mycobacterium tuberculosis.Journal of clinical microbiology · 2000Article
- Ferric reduction is a potential iron acquisition mechanism for Histoplasma capsulatum.Infection and immunity · 1999Article
- Iron acquisition and metabolism by mycobacteria.Journal of bacteriology · 1999Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel extracellular mycobacterial enzyme was identified in the ruminant pathogen Mycobacterium paratuberculosis. The enzyme was capable of mobilizing iron from different sources such as ferric ammonium citrate, ferritin, and transferrin by reduction of the metal. The purified reductase had a calculated Mr of 17,000, was sensitive to proteinase K treatment, and had an isoelectric point of pH 9. Analysis of the amino acid composition revealed glycine, serine, asparagine (or aspartic acid), and glutamine (or glutamic acid) as the most frequently occurring residues. Enzymatic activity was highest at 37 degrees C and between pH 5 and 10. The calculated Km and Vmax for ferric ammonium citrate were 0.213 mM and 0.345 mM min(-1) mg(-1), respectively. Using a specific antireductase antibody in immunoelectron microscopy, we were able to detect the enzyme associated with intracellular mycobacteria in naturally M. paratuberculosis-infected bovine tissue. We prepose that the reductase of M. paratuberculosis represents an alternative strategy of mycobacteria to mobilize ferric iron and discuss its potential role in bacterial evasion of intracellular defense mechanisms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.