ArticleInfection and immunity1998
Identification and characterization of the PutP proline permease that contributes to in vivo survival of Staphylococcus aureus in animal models.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 82 citations in OpenAlex.
- Article
- Proline transporters ProT and PutP are required for Staphylococcus aureus infection.PLoS pathogens · 2023Article
- Proline Transport and Growth Changes in Proline Transport Mutants ofMicroorganisms · 2022Article
- Role of Proline in Pathogen and Host Interactions.Antioxidants & redox signaling · 2019Review
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- Article
- Article
- Catabolism of host-derived compounds during extracellular bacterial infections.Journal of cellular biochemistry · 2014Article
- Use of recombinase-based in vivo expression technology to characterize Enterococcus faecalis gene expression during infection identifies in vivo-expressed antisense RNAs and implicates the protease Eep in pathogenesis.Infection and immunity · 2012Article
- Contribution of amino acid catabolism to the tissue specific persistence of Campylobacter jejuni in a murine colonization model.PloS one · 2012Article
- The novel Candida albicans transporter Dur31 Is a multi-stage pathogenicity factor.PLoS pathogens · 2012Article
- Mutational and transcriptional analyses of the Staphylococcus aureus low-affinity proline transporter OpuD during in vitro growth and infection of murine tissues.FEMS immunology and medical microbiology · 2011Article
- Tricarboxylic acid cycle-dependent attenuation of Staphylococcus aureus in vivo virulence by selective inhibition of amino acid transport.Infection and immunity · 2009Article
- Glycine betaine uptake by the ProXVWZ ABC transporter contributes to the ability of Mycobacterium tuberculosis to initiate growth in human macrophages.Journal of bacteriology · 2008Article
- Transcriptional activation of the Staphylococcus aureus putP gene by low-proline-high osmotic conditions and during infection of murine and human tissues.Infection and immunity · 2006Article
- Secondary transport of amino acids in prokaryotes.The Journal of membrane biology · 2006Review
- Staphylococcus aureus virulence genes identified by bursa aurealis mutagenesis and nematode killing.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- phgABC, a three-gene operon required for growth of Streptococcus pneumoniae in hyperosmotic medium and in vivo.Infection and immunity · 2004Article
- PheP, a putative amino acid permease of Staphylococcus aureus, contributes to survival in vivo and during starvation.Infection and immunity · 2004Article
- Proline reverses the abnormal phenotypes of Colletotrichum trifolii associated with expression of endogenous constitutively active Ras.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is an important pathogen of humans and other animals, causing bacteremia, abscesses, endocarditis, and other infectious syndromes. A signature-tagged mutagenesis (STM) system was adapted for use in studying the genes required for in vivo survival of S. aureus. An STM library was ultimately created in S. aureus RN6390, with Tn917 being used to create the transposon mutations. Pools of S. aureus RN6390 mutants were screened in mouse abscess, bacteremia, and wound infection models for growth attenuation after in vivo passage. One of the mutants that was identified displayed marked attenuation following large-pool screening in all three animal models, which was confirmed in bacteremia and endocarditis models of infection with a smaller pool of mutants. Sequence analysis of the entire open reading frame showed a 99% identity to the high-affinity proline permease (putP) gene characterized in another strain of S. aureus. In wound and murine abscess infection models, the putP mutant was approximately 10-fold more attenuated than was wild-type strain RN6390. Another S. aureus strain transduced with the putP mutation also displayed an attenuated phenotype after passage in the wound model. A [3H]proline uptake assay showed that less proline was specifically transported into the putP mutant than into strain RN6390. The reduced viability of the bacteria possessing the mutation in the S. aureus high-affinity proline permease suggests that proline scavenging by the bacteria is important for in vivo growth and proliferation and that analogs of proline may serve as potential antistaphylococcal therapeutic agents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.