ArticleInfection and immunity1998
Transcellular passage of Neisseria gonorrhoeae involves pilus phase variation.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Publicly Available Neisseria Gonorrhoeae Genomes Predominantly Represent In Vitro-Derived Nonpiliated Variants.The Journal of infectious diseases · 2026Article
- Internalization ofMicroorganisms · 2025Article
- Addressing Sexually Transmitted Infections Due toMicroorganisms · 2024Review
- Tissue Models forFrontiers in cellular and infection microbiology · 2022Review
- Anti-Virulence Therapeutic Approaches forAntibiotics (Basel, Switzerland) · 2021Review
- Inactivation of Genes Encoding MutL and MutS Proteins Influences Adhesion and Biofilm Formation byMicroorganisms · 2019Article
- Neisseria gonorrhoeae infects the human endocervix by activating non-muscle myosin II-mediated epithelial exfoliation.PLoS pathogens · 2017Article
- Expression of Opacity Proteins Interferes with the Transmigration of Neisseria gonorrhoeae across Polarized Epithelial Cells.PloS one · 2015Article
- Neisseria gonorrhoeae breaches the apical junction of polarized epithelial cells for transmigration by activating EGFR.Cellular microbiology · 2013Article
- Pilus phase variation switches gonococcal adherence to invasion by caveolin-1-dependent host cell signaling.PLoS pathogens · 2013Article
- The Pathobiology of Neisseria gonorrhoeae Lower Female Genital Tract Infection.Frontiers in microbiology · 2011Article
- Transcellular passage of Neisseria meningitidis across a polarized respiratory epithelium.Infection and immunity · 2010Article
- Imaging of disease dynamics during meningococcal sepsis.PloS one · 2007Article
- Gonococci exit apically and basally from polarized epithelial cells and exhibit dynamic changes in type IV pili.Cellular microbiology · 2006Article
- Global gene expression and the role of sigma factors in Neisseria gonorrhoeae in interactions with epithelial cells.Infection and immunity · 2005Article
- Soluble pilin of Neisseria gonorrhoeae interacts with human target cells and tissue.Infection and immunity · 2001Article
- Effect of alpha-oligosaccharide phenotype of Neisseria gonorrhoeae strain MS11 on invasion of Chang conjunctival, HEC-1-B endometrial, and ME-180 cervical cells.Infection and immunity · 2000Article
- Role of ribosomal protein L12 in gonococcal invasion of Hec1B cells.Infection and immunity · 2000Article
- Neisseria gonorrhoeae coordinately uses Pili and Opa to activate HEC-1-B cell microvilli, which causes engulfment of the gonococci.Infection and immunity · 1999Article
- Invasion of human mucosal epithelial cells by Neisseria gonorrhoeae upregulates expression of intercellular adhesion molecule 1 (ICAM-1).Infection and immunity · 1999Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Piliated and nonpiliated Neisseria gonorrhoeae organisms were added on top of confluent layers of HEC-1-B cells, each maintained on a microporous Transwell-COL membrane. The bacteria released into the lower chamber were characterized with respect to the following virulence determinants: pili, which mediate adherence to target host cells; PilE, the major pilus subunit protein; and PilC, which is involved in pilus biogenesis and adherence. Even if >99% of the added bacteria of N. gonorrhoeae MS11 were piliated, bacteria recovered on the other side of the cell layer were predominantly nonpiliated. The recovered clones still expressed unassembled PilE protein, but 50% had lost PilC production. Nonpiliated gonococci, in which the 5' end of pilE had been deleted, were released in reduced numbers, and piliated recA bacteria added to the cell layer were not released at all, at time points when piliated recA+ clones were found at high numbers in the lower chamber. Our data indicate that bacteria producing unassembled PilE protein are selected for during passage through an epithelial cell layer. The finding that the pilE gene sequence was altered in the transmigrants suggests that pilin sequence variation is involved in the transcellular passage of N. gonorrhoeae.
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Registered trials
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