ArticleThe EMBO journal1998
Essential role of tubulin-folding cofactor D in microtubule assembly and its association with microtubules in fission yeast.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Chromosome territories and the global regulation of the genome.Genes, chromosomes & cancer · 2019Review
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- Nucleotide Binding to ARL2 in the TBCD∙ARL2∙β-Tubulin Complex Drives Conformational Changes in β-Tubulin.Journal of molecular biology · 2017Article
- A Trimer Consisting of the Tubulin-specific Chaperone D (TBCD), Regulatory GTPase ARL2, and β-Tubulin Is Required for Maintaining the Microtubule Network.The Journal of biological chemistry · 2017Article
- Revisiting the tubulin cofactors and Arl2 in the regulation of soluble αβ-tubulin pools and their effect on microtubule dynamics.Molecular biology of the cell · 2017Article
- Infantile neurodegenerative disorder associated with mutations in TBCD, an essential gene in the tubulin heterodimer assembly pathway.Human molecular genetics · 2016Article
- Higher order signaling: ARL2 as regulator of both mitochondrial fusion and microtubule dynamics allows integration of 2 essential cell functions.Small GTPases · 2016Review
- Large-scale probabilistic 3D organization of human chromosome territories.Human molecular genetics · 2016Article
- A STRIPAK component Strip regulates neuronal morphogenesis by affecting microtubule stability.Scientific reports · 2015Article
- Article
- The dual role of fission yeast Tbc1/cofactor C orchestrates microtubule homeostasis in tubulin folding and acts as a GAP for GTPase Alp41/Arl2.Molecular biology of the cell · 2013Article
- Model of fission yeast cell shape driven by membrane-bound growth factors and the cytoskeleton.PLoS computational biology · 2013Article
- Faithful chaperones.Cellular and molecular life sciences : CMLS · 2011Review
- Effect of TBCD and its regulatory interactor Arl2 on tubulin and microtubule integrity.Cytoskeleton (Hoboken, N.J.) · 2010Article
- TBCD links centriologenesis, spindle microtubule dynamics, and midbody abscission in human cells.PloS one · 2010Article
- Shaping fission yeast with microtubules.Cold Spring Harbor perspectives in biology · 2009Review
- Tubulin heterodimers remain functional for one cell cycle after the inactivation of tubulin-folding cofactor D in fission yeast cells.Yeast (Chichester, England) · 2009Article
- Chromosome segregation in fission yeast with mutations in the tubulin folding cofactor D.Current genetics · 2006Article
- Fission yeast MO25 protein is localized at SPB and septum and is essential for cell morphogenesis.The EMBO journal · 2005Article
- Plant-specific microtubule-associated protein SPIRAL2 is required for anisotropic growth in Arabidopsis.Plant physiology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The main structural components of microtubules are alpha- and beta-tubulins. A group of proteins called cofactors are crucial in the formation of assembly-competent tubulin molecules in vitro. Whilst an in vitro role is emerging for these cofactors, their biological functions in vivo remain to be established. In order to understand the fundamental mechanisms that determine cell polarity, we have screened for fission yeast mutants with altered polarity. Here we show that alp1+ encodes a homologue of cofactor D and executes a function essential for cell viability. A temperature-sensitive alp1 mutant shows a variety of defects including abnormal mitoses, loss of microtubule structures, displacement of the nucleus, altered growth polarity and asymmetrical cell division. Overexpression of Alp1 is lethal in wild-type cells, resulting in altered cell shape, but is rescued by co-overexpression of beta-tubulin. Alp1 co-localizes with microtubules, both interphase arrays and mitotic spindles. Furthermore, Alp1 binds to and co-sediments with taxol (paclitaxel)-stabilized porcine microtubules. Our results suggest that, in addition to a function in the folding of beta-tubulin, cofactor D may play a vital role in microtubule-dependent processes as a microtubule-associated protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.