ArticleMolecular biology of the cell1998
Wortmannin-sensitive phosphorylation, translocation, and activation of PLCgamma1, but not PLCgamma2, in antigen-stimulated RBL-2H3 mast cells.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 102 citations in OpenAlex.
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- Endocytosis and the internalization of pathogenic organisms: focus on phosphoinositides.F1000Research · 2020Review
- Protein kinase D at the Golgi controls NLRP3 inflammasome activation.The Journal of experimental medicine · 2017Article
- The FcεRI Signaling Cascade and Integrin Trafficking Converge at Patterned Ligand Surfaces.Molecular biology of the cell · 2017Article
- Optimal aggregation of FcεRI with a structurally defined trivalent ligand overrides negative regulation driven by phosphatases.ACS chemical biology · 2014Article
- An Interaction Library for the FcεRI Signaling Network.Frontiers in immunology · 2014Review
- Inhibitors of PI(4,5)P2 synthesis reveal dynamic regulation of IgE receptor signaling by phosphoinositides in RBL mast cells.Molecular pharmacology · 2013Article
- Toxoplasma gondii inhibits mast cell degranulation by suppressing phospholipase Cγ-mediated Ca(2+) mobilization.Frontiers in microbiology · 2013Article
- LAB/NTAL/Lat2: a force to be reckoned with in all leukocytes?Journal of leukocyte biology · 2011Review
- Regulation of mast cell responses in health and disease.Critical reviews in immunology · 2011Review
- Single-cell analysis of phosphoinositide 3-kinase and phosphatase and tensin homolog activation.Faraday discussions · 2011Article
- Sprouty proteins inhibit receptor-mediated activation of phosphatidylinositol-specific phospholipase C.Molecular biology of the cell · 2010Article
- Functional features of gene expression profiles differentiating gastrointestinal stromal tumours according to KIT mutations and expression.BMC cancer · 2009Article
- FcepsilonRI-induced activation by low antigen concentrations results in nuclear signals in the absence of degranulation.Molecular immunology · 2009Article
- Restricted epithelial proliferation by lacritin via PKCalpha-dependent NFAT and mTOR pathways.The Journal of cell biology · 2006Article
- Reaction diffusion modeling of calcium dynamics with realistic ER geometry.Biophysical journal · 2006Article
- PKD2 functions as an epidermal growth factor-activated plasma membrane channel.Molecular and cellular biology · 2005Article
- Inhibition of the antigen-induced activation of rodent mast cells by putative Janus kinase 3 inhibitors WHI-P131 and WHI-P154 in a Janus kinase 3-independent manner.British journal of pharmacology · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In RBL-2H3 tumor mast cells, cross-linking the high affinity IgE receptor (FcepsilonRI) with antigen activates cytosolic tyrosine kinases and stimulates Ins(1,4,5)P3 production. Using immune complex phospholipase assays, we show that FcepsilonRI cross-linking activates both PLCgamma1 and PLCgamma2. Activation is accompanied by the increased phosphorylation of both PLCgamma isoforms on serine and tyrosine in antigen-treated cells. We also show that the two PLCgamma isoforms have distinct subcellular localizations. PLCgamma1 is primarily cytosolic in resting RBL-2H3 cells, with low levels of plasma membrane association. After antigen stimulation, PLCgamma1 translocates to the plasma membrane where it associates preferentially with membrane ruffles. In contrast, PLCgamma2 is concentrated in a perinuclear region near the Golgi and adjacent to the plasma membrane in resting cells and does not redistribute appreciably after FcepsilonRI cross-linking. The activation of PLCgamma1, but not of PLCgamma2, is blocked by wortmannin, a PI 3-kinase inhibitor previously shown to block antigen-stimulated ruffling and to inhibit Ins(1,4,5)P3 synthesis. In addition, wortmannin strongly inhibits the antigen-stimulated phosphorylation of both serine and tyrosine residues on PLCgamma1 with little inhibition of PLCgamma2 phosphorylation. Wortmannin also blocks the antigen-stimulated translocation of PLCgamma1 to the plasma membrane. Our results implicate PI 3-kinase in the phosphorylation, translocation, and activation of PLCgamma1. Although less abundant than PLCgamma2, activated PLCgamma1 may be responsible for the bulk of antigen-stimulated Ins(1,4,5)P3 production in RBL-2H3 cells.
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