ArticleMolecular biology of the cell1998
Identification of a human VPF/VEGF 3' untranslated region mediating hypoxia-induced mRNA stability.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 179 citations in OpenAlex.
- Analysis of RNA Interference Targeted Against Human Antigen R (HuR) to Reduce Vascular Endothelial Growth Factor (VEGF) Protein Expression in Human Retinal Pigment Epithelial Cells.Molecular biotechnology · 2024Article
- Crosstalk between phytochemicals and inflammatory signaling pathways.Inflammopharmacology · 2023Review
- Review
- Calreticulin regulates vascular endothelial growth factor-A mRNA stability in gastric cancer cells.PloS one · 2019Article
- Secretion of VEGF-165 has unique characteristics, including shedding from the plasma membrane.Molecular biology of the cell · 2014Article
- Suppression of laser-induced choroidal neovascularization by intravitreal injection of tristetraprolin.International journal of ophthalmology · 2014Article
- A miR-297/hypoxia/DGK-α axis regulating glioblastoma survival.Neuro-oncology · 2013Article
- The novel tumor suppressor NOL7 post-transcriptionally regulates thrombospondin-1 expression.Oncogene · 2013Article
- VEGF-A mRNA processing, stability and translation: a paradigm for intricate regulation of gene expression at the post-transcriptional level.Nucleic acids research · 2013Review
- Identification of DEAD-box RNA helicase 6 (DDX6) as a cellular modulator of vascular endothelial growth factor expression under hypoxia.The Journal of biological chemistry · 2013Article
- Antagonistic function of the RNA-binding protein HuR and miR-200b in post-transcriptional regulation of vascular endothelial growth factor-A expression and angiogenesis.The Journal of biological chemistry · 2013Article
- miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish.Development (Cambridge, England) · 2012Article
- The expression levels of microRNA-361-5p and its target VEGFA are inversely correlated in human cutaneous squamous cell carcinoma.PloS one · 2012Article
- MDM2 regulates vascular endothelial growth factor mRNA stabilization in hypoxia.Molecular and cellular biology · 2011Article
- Repression of VEGFA by CA-rich element-binding microRNAs is modulated by hnRNP L.The EMBO journal · 2011Article
- Post-transcriptional control during chronic inflammation and cancer: a focus on AU-rich elements.Cellular and molecular life sciences : CMLS · 2010Review
- Extracellular ATP may contribute to tissue repair by rapidly stimulating purinergic receptor X7-dependent vascular endothelial growth factor release from primary human monocytes.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Astrocyte-derived vascular endothelial growth factor stabilizes vessels in the developing retinal vasculature.PloS one · 2010Article
- Article
- FoxM1B transcriptionally regulates vascular endothelial growth factor expression and promotes the angiogenesis and growth of glioma cells.Cancer research · 2008Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Hypoxia is a prominent feature of malignant tumors that are characterized by angiogenesis and vascular hyperpermeability. Vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) has been shown to be up-regulated in the vicinity of necrotic tumor areas, and hypoxia potently induces VPF/VEGF expression in several tumor cell lines in vitro. Here we report that hypoxia-induced VPF/VEGF expression is mediated by increased transcription and mRNA stability in human M21 melanoma cells. RNA-binding/electrophoretic mobility shift assays identified a single 125-bp AU-rich element in the 3' untranslated region that formed hypoxia-inducible RNA-protein complexes. Hypoxia-induced expression of chimeric luciferase reporter constructs containing this 125-bp AU-rich hypoxia stability region were significantly higher than constructs containing an adjacent 3' untranslated region element without RNA-binding activity. Using UV-cross-linking studies, we have identified a series of hypoxia-induced proteins of 90/88 kDa, 72 kDa, 60 kDa, 56 kDa, and 46 kDa that bound to the hypoxia stability region element. The 90/88-kDa and 60-kDa species were specifically competed by excess hypoxia stability region RNA. Thus, increased VPF/VEGF mRNA stability induced by hypoxia is mediated, at least in part, by specific interactions between a defined mRNA stability sequence in the 3' untranslated region and distinct mRNA-binding proteins in human tumor cells.
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Registered trials
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