ArticleMolecular biology of the cell1998
Inhibition of endosome fusion by wortmannin persists in the presence of activated Rab5.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 48 citations in OpenAlex.
- Rab21 recruits EEA1 and competes with Rab5 for Rabex-5 activation.Frontiers in cell and developmental biology · 2025Article
- Phosphatidylinositol 3-kinase and Rab5 GTPase inversely regulate the Smad anchor for receptor activation (SARA) protein independently of transforming growth factor-β1.The Journal of biological chemistry · 2012Article
- Wortmannin induces homotypic fusion of plant prevacuolar compartments.Journal of experimental botany · 2009Article
- SNARE function is not involved in early endosome docking.Molecular biology of the cell · 2008Article
- Endocytosis in the shiitake mushroom Lentinula edodes and involvement of GTPase LeRAB7.Eukaryotic cell · 2007Article
- Rice SCAMP1 defines clathrin-coated, trans-golgi-located tubular-vesicular structures as an early endosome in tobacco BY-2 cells.The Plant cell · 2007Article
- Early events in integrin alphavbeta6-mediated cell entry of foot-and-mouth disease virus.Journal of virology · 2005Article
- The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane.Molecular biology of the cell · 2005Article
- Xanthine nucleotide-specific G-protein alpha-subunits: a novel approach for the analysis of G-protein-mediated signal transduction.Naunyn-Schmiedeberg's archives of pharmacology · 2004Review
- Phagosome maturation: aging gracefully.The Biochemical journal · 2002Review
- The cytoplasmic tail of invariant chain regulates endosome fusion and morphology.Molecular biology of the cell · 2002Article
- Inhibition of Na+-H+ exchanger-3 interferes with apical receptor-mediated endocytosis via vesicle fusion.The Journal of physiology · 2001Article
- Trafficking of phosphatidylinositol 3-phosphate from the trans-Golgi network to the lumen of the central vacuole in plant cells.The Plant cell · 2001Article
- Regulation of intracellular trafficking of the EGF receptor by Rab5 in the absence of phosphatidylinositol 3-kinase activity.EMBO reports · 2001Article
- Tethering molecules in membrane traffic.Protoplasma · 1999Article
- Molecular aspects of the endocytic pathway.The Biochemical journal · 1998Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Rab5-dependent endosome fusion is sensitive to the phosphoinositide 3-kinase inhibitor, wortmannin. It has been proposed that phosphoinositide 3-kinase activity may be required for activation of rab5 by influencing its nucleotide cycle such as to promote its active GTP state. In this report we demonstrate that endosome fusion remains sensitive to wortmannin despite preloading of endosomes with stimulatory levels of a GTPase-defective mutant rab5(Q79L) or of a xanthosine triphosphate-binding mutant, rab5(D136N), in the presence of the nonhydrolysable analogue XTPgammaS. These results suggest that activation of rab5 cannot be the principal function of the wortmannin-sensitive factor on the endosome fusion pathway. This result is extrapolated to all GTPases by demonstrating that endosome fusion remains wortmannin sensitive despite prior incubation with the nonhydrolysable nucleotide analogue GTPgammaS. Consistent with these results, direct measurement of clathrin-coated vesicle-stimulated nucleotide dissociation from exogenous rab5 was insensitive to the presence of wortmannin. A large excess of rab5(Q79L), beyond levels required for maximal stimulation of the fusion assay, afforded protection against wortmannin inhibition, and partial protection was also observed with an excess of wild-type rab5 independent of GTPgammaS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.