ArticleMolecular biology of the cell1998
Targeting of a germ cell-specific type 1 hexokinase lacking a porin-binding domain to the mitochondria as well as to the head and fibrous sheath of murine spermatozoa.
Article in Molecular biology of the cell, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 136 citations in OpenAlex.
- Trial
- Mitochonic Acid 5 Increases Ram Sperm Quality by Improving Mitochondrial Function during Storage at 4 °C.Animals : an open access journal from MDPI · 2024Article
- Nomograms for Predicting Postoperative Sperm Improvements in Varicocele Patients.European urology open science · 2024Article
- Expanding the Molecular Spectrum ofArchives of Iranian medicine · 2023Article
- The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C.Animals : an open access journal from MDPI · 2022Article
- Pathophysiology of Mitochondrial Dysfunction in Human Spermatozoa: Focus on Energetic Metabolism, Oxidative Stress and Apoptosis.Antioxidants (Basel, Switzerland) · 2021Review
- Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility.Journal of clinical medicine · 2020Review
- A trade-off between thickness and length in the zebra finch sperm mid-piece.Proceedings. Biological sciences · 2018Article
- Advanced Paternal Age Affects Sperm Count and Anogenital Distance in Mouse Offspring.Reproductive sciences (Thousand Oaks, Calif.) · 2018Article
- Gel electrophoresis of human sperm: a simple method for evaluating sperm protein quality.Basic and clinical andrology · 2018Article
- The Stimulated Glycolytic Pathway Is Able to Maintain ATP Levels and Kinetic Patterns of Bovine Epididymal Sperm Subjected to Mitochondrial Uncoupling.Oxidative medicine and cellular longevity · 2017Article
- Novel myristoylation of the sperm-specific hexokinase 1 isoform regulates its atypical localization.Biology open · 2015Article
- Article
- Mass-Specific Metabolic Rate Influences Sperm Performance through Energy Production in Mammals.PloS one · 2015Article
- Male mice express spermatogenic cell-specific triosephosphate isomerase isozymes.Molecular reproduction and development · 2013Article
- Phospholipase B is activated in response to sterol removal and stimulates acrosome exocytosis in murine sperm.The Journal of biological chemistry · 2013Article
- Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker.Journal of assisted reproduction and genetics · 2013Article
- Biomimicry enhances sequential reactions of tethered glycolytic enzymes, TPI and GAPDHS.PloS one · 2013Article
- Lactotransferrin in Asian elephant (Elephas maximus) seminal plasma correlates with semen quality.PloS one · 2013Article
- What sperm can teach us about energy production.Reproduction in domestic animals = Zuchthygiene · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Multiple isoforms of type 1 hexokinase (HK1) are transcribed during spermatogenesis in the mouse, including at least three that are presumably germ cell specific: HK1-sa, HK1-sb, and HK1-sc. Each of these predicted proteins contains a common, germ cell-specific sequence that replaces the porin-binding domain found in somatic HK1. Although HK1 protein is present in mature sperm and is tyrosine phosphorylated, it is not known whether the various potential isoforms are differentially translated and localized within the developing germ cells and mature sperm. Using antipeptide antisera against unique regions of HK1-sa and HK1-sb, it was demonstrated that these isoforms were not found in pachytene spermatocytes, round spermatids, condensing spermatids, or sperm, suggesting that HK1-sa and HK1-sb are not translated during spermatogenesis. Immunoreactivity was detected in protein from round spermatids, condensing spermatids, and mature sperm using an antipeptide antiserum against the common, germ cell-specific region, suggesting that HK1-sc was the only germ cell-specific isoform present in these cells. Two-dimensional SDS-PAGE suggested that all of the sperm HK1-sc was tyrosine phosphorylated, and that the somatic HK1 isoform was not present. Immunoelectron microscopy revealed that HK1-sc was associated with the mitochondria and with the fibrous sheath of the flagellum and was found in discrete clusters in the region of the membranes of the sperm head. The unusual distribution of HK1-sc in sperm suggests novel functions, such as extramitochondrial energy production, and also demonstrates that a hexokinase without a classical porin-binding domain can localize to mitochondria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.