ArticleThe Journal of clinical investigation1998
Reduced extracellular pH increases endothelin-1 secretion by human renal microvascular endothelial cells.
Article in The Journal of clinical investigation, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 74 citations in OpenAlex.
- Metabolic acidosis in chronic kidney disease: mere consequence or also culprit?Pflugers Archiv : European journal of physiology · 2024Review
- Endothelin Receptor Antagonists: Status Quo and Future Perspectives for Targeted Therapy.Journal of clinical medicine · 2020Review
- Chronic lithium treatment induces novel patterns of pendrin localization and expression.American journal of physiology. Renal physiology · 2018Article
- Endothelial cells enhance the migration of bovine meniscus cells.Arthritis & rheumatology (Hoboken, N.J.) · 2015Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Potential approaches to reverse or repair renal fibrosis.Nature reviews. Nephrology · 2014Review
- Endothelin-1 and the kidney--beyond BP.British journal of pharmacology · 2012Review
- CFTR regulation of intracellular pH and ceramides is required for lung endothelial cell apoptosis.American journal of respiratory cell and molecular biology · 2009Article
- Increased endothelin activity mediates augmented distal nephron acidification induced by dietary protein.Transactions of the American Clinical and Climatological Association · 2005Article
- Acid sensing in renal epithelial cells.The Journal of clinical investigation · 2004Review
- Endothelial dysfunction and atherosclerosis: endothelin receptor antagonists as novel therapeutics.Current hypertension reports · 2000Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Because dietary acid increases renal secretion of endothelin-1 (ET-1) which is synthesized by renal microvascular endothelium, we examined if reduced extracellular pH increases ET-1 secretion by cultured human renal microvascular endothelial cells (RMVECs). Confluent cells were exposed to serum-free media for 24 h, then incubated in either control, acid, or alkaline serum-free media for 12 h. Standard growth media pH was 7.2 after equilibration with 5% CO2 at 37 degrees C and was made the pH of control media. Acid and alkaline media pH were 7.0 and 7.4, respectively. Added Hepes and Tris maintained all assigned pHs. Media ET-1 measured by RIA after column extraction was higher for RMVECs exposed to acid compared with control media (170.0+/-17.1 vs. 64.6+/-9.6 pM, P < 0.004) but those exposed to alkaline media (56.6+/-25.1 pM, P = NS vs. control) were not. Human aortic endothelial cells exposed to control, acid, and alkaline media had similar ET-1 (166.6+/-18.1, 139.3+/-18.5, and 205.9+/-25.3 pM, P = NS). The data show acid-stimulated ET-1 secretion by RMVECs but not aortic endothelial cells, demonstrating a new environmental factor that influences ET-1 secretion by renal microvascular endothelium and thereby possibly modulates endothelin-dependent processes in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.