ArticleBiophysical journal1998
Determining force dependence of two-dimensional receptor-ligand binding affinity by centrifugation.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
52 citing papers in PubMed.
- Mechanotransduction through T cell receptors: consensus, controversies and future outlooks.Experimental & molecular medicine · 2026Review
- Mechanotransduction governs CD40 function and underlies X-linked hyper-IgM syndrome.Science advances · 2024Article
- Effect of Circadian Rhythm Modulated Blood Flow on Nanoparticle based Targeted Drug Delivery in VirtualbioRxiv : the preprint server for biology · 2024Article
- Chimeric nanobody-decorated liposomes by self-assembly.Nature nanotechnology · 2024Article
- Modeling of spatiotemporal dynamics of ligand-coated particle flow in targeted drug delivery processes.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Micropipette-based biomechanical nanotools on living cells.European biophysics journal : EBJ · 2022Review
- Integrin Regulated Autoimmune Disorders: Understanding the Role of Mechanical Force in Autoimmunity.Frontiers in cell and developmental biology · 2022Review
- Single-cell measurements of two-dimensional binding affinity across cell contacts.Biophysical journal · 2021Article
- Computational and experimental investigation of particulate matter deposition in cerebral side aneurysms.Journal of the Royal Society, Interface · 2020Article
- Temperature-Induced Catch-Slip to Slip Bond Transit in Plasmodium falciparum-Infected Erythrocytes.Biophysical journal · 2020Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- A microfluidic technique to estimate antigen expression on particles.APL bioengineering · 2017Article
- A point-of-care microfluidic biochip for quantification of CD64 expression from whole blood for sepsis stratification.Nature communications · 2017Article
- Single molecule and multiple bond characterization of catch bond associated cytoadhesion in malaria.Scientific reports · 2017Article
- Binding equilibrium and kinetics of membrane-anchored receptors and ligands in cell adhesion: Insights from computational model systems and theory.Cell adhesion & migration · 2016Review
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- Particle shape enhances specificity of antibody-displaying nanoparticles.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Biodegradable nanoparticles mimicking platelet binding as a targeted and controlled drug delivery system.International journal of pharmaceutics · 2012Article
- Rapid tumoritropic accumulation of systemically injected plateloid particles and their biodistribution.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Analyses of receptor-ligand interactions are important to the understanding of cellular adhesion. Traditional methods of measuring the three-dimensional (3D) dissociation constant (Kd) require at least one of the molecular species in solution and hence cannot be directly applied to the case of cell adhesion. We describe a novel method of measuring 2D binding characteristics of receptors and ligands that are attached to surfaces and whose bonds are subjected to forces. The method utilizes a common centrifugation assay to quantify adhesion. A model for the experiment has been formulated, solved exactly, and tested carefully. The model is stochastically based and couples the bond force to the binding affinity. The method was applied to examine tumor cell adherence to recombinant E-selectin. Satisfactory agreement was found between predictions and data. The estimated zero-force 2D Kd for E-selectin/carbohydrate ligand binding was approximately 5 x 10(3) microm(-2), and the bond interaction range was subangstrom. Our results also suggest that the number of bonds mediating adhesion was small (<5).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.