ArticleBiophysical journal1998
Thermal imaging of receptor-activated heat production in single cells.
Article in Biophysical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 181 citations in OpenAlex.
- Luminescent Lanthanides in Biorelated Applications: From Molecules to Nanoparticles and Diagnostic Probes to Therapeutics.Chemical reviews · 2025Review
- Temperature imaging inside fluid devices using a ratiometric near infrared (NIR-II/III) fluorescent YAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Nanoscale thermal control of a single living cell enabled by diamond heater-thermometer.Scientific reports · 2023Article
- How sticky? How tight? How hot? Imaging probes for fluid viscosity, membrane tension and temperature measurements at the cellular level.The international journal of biochemistry & cell biology · 2022Review
- Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer.Nano letters · 2022Article
- Thermal Probing Techniques for a Single Live Cell.Sensors (Basel, Switzerland) · 2022Review
- Perspective: a stirring role for metabolism in cells.Molecular systems biology · 2022Review
- Intracellular thermometry uncovers spontaneous thermogenesis and associated thermal signaling.Communications biology · 2021Review
- In Situ Synthesis of β-NaMolecules (Basel, Switzerland) · 2021Article
- Luminescent YbNanomaterials (Basel, Switzerland) · 2021Article
- Single-cell temperature mapping with fluorescent thermometer nanosheets.The Journal of general physiology · 2020Article
- How hot are single cells?The Journal of general physiology · 2020Article
- Bifunctional TmNanomaterials (Basel, Switzerland) · 2020Article
- GHz Ultrasonic Chip-Scale Device Induces Ion Channel Stimulation in Human Neural Cells.Scientific reports · 2020Article
- Modeling of an Optically Heated MEMS-Based Micromechanical Bimaterial Sensor for Heat Capacitance Measurements of Single Biological Cells.Sensors (Basel, Switzerland) · 2019Article
- Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy.Nature protocols · 2019Article
- Frequency-induced morphology alterations in microconfined biological cells.Medical & biological engineering & computing · 2019Article
- Intracellular ratiometric temperature sensing using fluorescent carbon dots.Nanoscale advances · 2019Article
- High-performance flexible metal-on-silicon thermocouple.Scientific reports · 2018Article
- Ratiometric nanothermometer in vivo based on triplet sensitized upconversion.Nature communications · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Changes in enthalpy (i.e., heat content) occur during the diverse intracellular chemical and biophysical interactions that take place in the life cycle of biological cells. Such changes have previously been measured for cell suspensions or cell-free biochemical extracts by using microcalorimetry, thermocouples, or pyroelectric films, all of which afford minimal spatial or temporal resolution. Here we present a novel thermal imaging method that combines both diffraction-limited spatial (approximately 300 nm) and sampling-rate-limited time resolution, using the temperature-dependent phosphorescence intensity of the rare earth chelate Eu-TTA (europium (III) thenoyltrifluoro-acetonate). With this thermosensitive dye, we imaged intracellular heat waves evoked in Chinese hamster ovary cells after activation of the metabotropic m1-muscarinic receptor. Fast application of acetylcholine onto the cells evoked a biphasic heat wave that was blocked by atropine, and after a brief delay was followed by a calcium wave. Atropine applied by itself produced a monophasic heat wave in the cells, suggesting that its interactions with the receptor activate some intracellular metabolic pathways. The thermal imaging technique introduced here should provide new insights into cellular functions by resolving the location, kinetics, and quantity of intracellular heat production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.