ArticleProceedings of the National Academy of Sciences of the United States of America1998
X-ray-induced mutations in mouse embryonic stem cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 62 citations in OpenAlex.
- Regional random mutagenesis driven by multiple sgRNAs and diverse on-target genome editing events to identify functionally important elements in non-coding regions.Open biology · 2024Article
- Identification of Nanog as a novel inhibitor of Rad51.Cell death & disease · 2022Article
- Article
- LIG4 mediates Wnt signalling-induced radioresistance.Nature communications · 2016Article
- Ionizing Radiation Impacts on Cardiac Differentiation of Mouse Embryonic Stem Cells.Stem cells and development · 2016Article
- Human induced pluripotent cells resemble embryonic stem cells demonstrating enhanced levels of DNA repair and efficacy of nonhomologous end-joining.Mutation research · 2011Article
- DNA repair: the culprit for tumor-initiating cell survival?Cancer metastasis reviews · 2011Review
- Creating a "hopeful monster": mouse forward genetic screens.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Spontaneous and x-ray-triggered crystallization at long range in self-assembling filament networks.Science (New York, N.Y.) · 2010Article
- DNA repair in murine embryonic stem cells and differentiated cells.Experimental cell research · 2008Review
- Mouse chromosome engineering for modeling human disease.Annual review of genomics and human genetics · 2006Review
- X-ray-induced deletion complexes in embryonic stem cells on mouse chromosome 15.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- Genomic instability in radial growth phase melanoma cell lines after ultraviolet irradiation.Journal of clinical pathology · 2005Article
- Moving forward with chemical mutagenesis in the mouse.The Journal of physiology · 2004Review
- Development of an enhanced GFP-based dual-color reporter to facilitate genetic screens for the recovery of mutations in mice.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Human umbilical cord blood (HUCB) cells for central nervous system repair.Neurotoxicity research · 2003Review
- Article
- Functional annotation of mammalian genomic DNA sequence by chemical mutagenesis: a fine-structure genetic mutation map of a 1- to 2-cM segment of mouse chromosome 7 corresponding to human chromosome 11p14-p15.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Molecular and functional mapping of the piebald deletion complex on mouse chromosome 14.Genetics · 2001Article
- Rapid generation of nested chromosomal deletions on mouse chromosome 2.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Deletion complexes consisting of multiple chromosomal deletions induced at single loci can provide a means for functional analysis of regions spanning several centimorgans in model genetic systems. A strategy to identify and map deletions at any cloned locus in the mouse is described here. First, a highly polymorphic, germ-line competent F1(129/Sv-+Tyr+p x CAST/Ei) mouse embryonic stem cell line was established. Then, x-ray and UV-induced mutagenesis was performed to determine the feasibility of generating deletion complexes throughout the mouse genome. Reported here are the selection protocols, induced mutation frequencies, cytogenetic and extensive molecular analysis of mutations at the X-chromosome-linked hypoxanthine phosphoribosyltransferase (Hprt) locus and at the neural cell adhesion molecule (Ncam) locus located on chromosome 9. Mutation analysis with PCR-based polymorphic microsatellite markers revealed deletions of <3 cM at the Hprt locus, whereas results consistent with deletions covering >28 cM were observed at the Ncam locus. Fluorescence in situ hybridization with a chromosome 9 paint revealed that some of the Ncam deletions were accompanied by complex chromosome rearrangements. In addition, deletion mapping in combination with loss of heterozygosity of microsatellite markers revealed a putative haploinsufficient region distal to Ncam. These data indicate that it is feasible to generate x-ray-induced deletion complexes in mouse embryonic stem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.