ArticleMolecular and cellular biology1998
Replication errors during in vivo Ty1 transposition are linked to heterogeneous RNase H cleavage sites.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 16 citations in OpenAlex.
- The self primer of the long terminal repeat retrotransposon Tf1 is not removed during reverse transcription.Journal of virology · 2006Article
- Specific recognition and cleavage of the plus-strand primer by reverse transcriptase.Journal of virology · 2005Article
- A nucleocapsid functionality contained within the amino terminus of the Ty1 protease that is distinct and separable from proteolytic activity.Journal of virology · 2002Article
- A Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerization.Journal of virology · 2001Article
- DNA synthesis fidelity by the reverse transcriptase of the yeast retrotransposon Ty1.Nucleic acids research · 2001Article
- In vivo Ty1 reverse transcription can generate replication intermediates with untidy ends.Journal of virology · 1998Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously identified a mutational hotspot upstream of the Ty1 U5-primer binding site (PBS) border and proposed a novel mechanism to account for this phenomenon during Ty1 replication. In this report, we verify key points of our model and show that in vivo RNase H cleavage of Ty1 RNA during minus-strand strong-stop synthesis creates heterogeneous 5' RNA ends. The preferred cleavage sites closest to the PBS are 6 and 3 bases upstream of the U5-PBS border. Minus-strand cDNA synthesis terminates at multiple sites determined by RNase H cleavage, and DNA intermediates frequently contain 3'-terminal sequence changes at or near their template ends. These data indicate that nontemplated terminal base addition during reverse transcription is a real in vivo phenomenon and suggest that this mechanism is a major source of sequence variability among retrotransposed genetic elements.
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Registered trials
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