ArticleMolecular and cellular biology1998
The yeast TAF145 inhibitory domain and TFIIA competitively bind to TATA-binding protein.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 119 citations in OpenAlex.
- The general transcription factors (GTFs) of RNA polymerase II and their roles in plant development and stress responses.Critical reviews in biochemistry and molecular biology · 2024Review
- Article
- IID in 3D: Improved Resolution of Transcription Factor Structure by Cryo-Electron Microscopy.Biochemistry · 2019Article
- Structure of human TFIID and mechanism of TBP loading onto promoter DNA.Science (New York, N.Y.) · 2018Article
- Towards a mechanistic understanding of core promoter recognition from cryo-EM studies of human TFIID.Current opinion in structural biology · 2017Review
- p53 Dynamically Directs TFIID Assembly on Target Gene Promoters.Molecular and cellular biology · 2017Article
- Structural Insights into the Eukaryotic Transcription Initiation Machinery.Annual review of biophysics · 2017Review
- Article
- Article
- Taspase1-dependent TFIIA cleavage coordinates head morphogenesis by limiting Cdkn2a locus transcription.The Journal of clinical investigation · 2015Article
- Structural basis of transcription initiation by RNA polymerase II.Nature reviews. Molecular cell biology · 2015Review
- Article
- Architecture of the Saccharomyces cerevisiae SAGA transcription coactivator complex.The EMBO journal · 2014Article
- Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways.Nucleic acids research · 2014Article
- Direct TFIIA-TFIID protein contacts drive budding yeast ribosomal protein gene transcription.The Journal of biological chemistry · 2013Article
- High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation.Nature structural & molecular biology · 2013Article
- Review
- TBP-related factors: a paradigm of diversity in transcription initiation.Cell & bioscience · 2011Article
- Molecular characterization of ScTFIIAgamma, encoding the putative TFIIA small subunit from sugarcane.Plant cell reports · 2010Article
- TFIIA and the transactivator Rap1 cooperate to commit TFIID for transcription initiation.Nature · 2010Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila 230-kDa TFIID subunit (dTAF230) interacts with the DNA binding domain of TATA box-binding protein (TBP) which exists in the same complex. Here, we characterize the inhibitory domain in the yeast TAF145 (yTAF145), which is homologous to dTAF230. Mutation studies show that the N-terminal inhibitory region (residues 10 to 71) can be divided into two subdomains, I (residues 10 to 37) and II (residues 46 to 71). Mutations in either subdomain significantly impair function. Acidic residues in subdomain II are important for the interaction with TBP. In addition, yTAF145 interaction is impaired by mutating the basic residues on the convex surface of TBP, which are crucial for interaction with TFIIA. Consistently, TFIIA and yTAF145 bind competitively to TBP. A deletion of the inhibitory domain of yTAF145 leads to a temperature-sensitive growth phenotype. Importantly, this phenotype is suppressed by overexpression of the TFIIA subunits, indicating that the yTAF145 inhibitory domain is involved in TFIIA function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.