ArticleMolecular and cellular biology1998
Two AAA family peroxins, PpPex1p and PpPex6p, interact with each other in an ATP-dependent manner and are associated with different subcellular membranous structures distinct from peroxisomes.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 139 citations in OpenAlex.
- Structure of the peroxisomal Pex1/Pex6 ATPase complex bound to a substrate.Nature communications · 2023Article
- The PEX1 ATPase Stabilizes PEX6 and Plays Essential Roles in Peroxisome Biology.Plant physiology · 2017Article
- Structure and Function of p97 and Pex1/6 Type II AAA+ Complexes.Frontiers in molecular biosciences · 2017Review
- Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae.Scientific reports · 2016Article
- Nucleotide-dependent assembly of the peroxisomal receptor export complex.Scientific reports · 2016Article
- Distinct requirements for intra-ER sorting and budding of peroxisomal membrane proteins from the ER.The Journal of cell biology · 2016Article
- Unique double-ring structure of the peroxisomal Pex1/Pex6 ATPase complex revealed by cryo-electron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The Pex1/Pex6 complex is a heterohexameric AAA+ motor with alternating and highly coordinated subunits.Journal of molecular biology · 2015Article
- AAA peroxins and their recruiter Pex26p modulate the interactions of peroxins involved in peroxisomal protein import.The Journal of biological chemistry · 2014Article
- Unique requirements for mono- and polyubiquitination of the peroxisomal targeting signal co-receptor, Pex20.The Journal of biological chemistry · 2013Article
- Arabidopsis ABERRANT PEROXISOME MORPHOLOGY9 is a peroxin that recruits the PEX1-PEX6 complex to peroxisomes.The Plant cell · 2011Article
- The peroxisomal matrix import of Pex8p requires only PTS receptors and Pex14p.Molecular biology of the cell · 2009Article
- Peroxisome matrix and membrane protein biogenesis.IUBMB life · 2009Review
- Dysferlin domain-containing proteins, Pex30p and Pex31p, localized to two compartments, control the number and size of oleate-induced peroxisomes in Pichia pastoris.Molecular biology of the cell · 2008Article
- The evolution of spinnable cotton fiber entailed prolonged development and a novel metabolism.PLoS genetics · 2008Article
- A conserved cysteine residue of Pichia pastoris Pex20p is essential for its recycling from the peroxisome to the cytosol.The Journal of biological chemistry · 2007Article
- Peroxisome function regulates growth on glucose in the basidiomycete fungus Cryptococcus neoformans.Eukaryotic cell · 2007Article
- Two independent pathways traffic the intraperoxisomal peroxin PpPex8p into peroxisomes: mechanism and evolutionary implications.Molecular biology of the cell · 2006Article
- Dynamics of the peroxisomal import cycle of PpPex20p: ubiquitin-dependent localization and regulation.The Journal of cell biology · 2006Article
- Alternative splicing suggests extended function of PEX26 in peroxisome biogenesis.American journal of human genetics · 2005Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Two peroxins of the AAA family, PpPex1p and PpPex6p, are required for peroxisome biogenesis in the yeast Pichia pastoris. Cells from the corresponding deletion strains (Pp delta pex1 and Pp delta pex6) contain only small vesicular remnants of peroxisomes, the bulk of peroxisomal matrix proteins is mislocalized to the cytosol, and these cells cannot grow in peroxisome-requiring media (J. A. Heyman, E. Monosov, and S. Subramani, J. Cell Biol. 127:1259-1273, 1994; A. P. Spong and S. Subramani, J. Cell Biol. 123:535-548, 1993). We demonstrate that PpPex1p and PpPex6p interact in an ATP-dependent manner. Genetically, the interaction was observed in a suppressor screen with a strain harboring a temperature-sensitive allele of PpPEX1 and in the yeast two-hybrid system. Biochemially, these proteins were coimmunoprecipitated with antibodies raised against either of the proteins, but only in the presence of ATP. The protein complex formed under these conditions was 320 to 400 kDa in size, consistent with the formation of a heterodimeric PpPex1p-PpPex6p complex. Subcellular fractionation revealed PpPex1p and PpPex6p to be predominantly associated with membranous subcellular structures distinct from peroxisomes. Based on their behavior in subcellular fractionation experiments including flotation gradients and on the fact that these structures are also present in a Pp delta pex3 strain in which no morphologically detectable peroxisomal remnants have been observed, we propose that these structures are small vesicles. The identification of vesicle-associated peroxins is novel and implies a role for these vesicles in peroxisome biogenesis. We discuss the possible role of the ATP-dependent interaction between PpPex1p and PpPex6p in regulating peroxisome biogenesis events.
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