ArticleMolecular and cellular biology1998
Interactions between Ty1 retrotransposon RNA and the T and D regions of the tRNA(iMet) primer are required for initiation of reverse transcription in vivo.
Article in Molecular and cellular biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- Cell Compartment-Specific Folding of Ty1 Long Terminal Repeat Retrotransposon RNA Genome.Viruses · 2022Article
- RNA Binding Properties of the Ty1 LTR-Retrotransposon Gag Protein.International journal of molecular sciences · 2021Article
- In vivo structure of the Ty1 retrotransposon RNA genome.Nucleic acids research · 2021Article
- Retroviral-like determinants and functions required for dimerization of Ty1 retrotransposon RNA.RNA biology · 2019Article
- Structure-Function Model for Kissing Loop Interactions That Initiate Dimerization of Ty1 RNA.Viruses · 2017Article
- Reverse Transcription in the Saccharomyces cerevisiae Long-Terminal Repeat Retrotransposon Ty3.Viruses · 2017Review
- Determinants of Genomic RNA Encapsidation in the Saccharomyces cerevisiae Long Terminal Repeat Retrotransposons Ty1 and Ty3.Viruses · 2016Review
- The Ty1 LTR-retrotransposon of budding yeast,Microbiology spectrum · 2015Article
- Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription.RNA (New York, N.Y.) · 2013Article
- Sequence requirements for localization and packaging of Ty3 retroelement RNA.Virus research · 2013Article
- Exploring Ty1 retrotransposon RNA structure within virus-like particles.Nucleic acids research · 2013Article
- Novel transcript truncating function of Rap1p revealed by synthetic codon-optimized Ty1 retrotransposon.Genetics · 2012Article
- Identification and characterization of critical cis-acting sequences within the yeast Ty1 retrotransposon.RNA (New York, N.Y.) · 2005Article
- Synthetic tRNALys,3 as the replication primer for the HIV-1HXB2 and HIV-1Mal genomes.Nucleic acids research · 2004Article
- Structure-function relationships of the initiation complex of HIV-1 reverse transcription: the case of mutant viruses using tRNA(His) as primer.Nucleic acids research · 2003Article
- On the importance of the primer activation signal for initiation of tRNA(lys3)-primed reverse transcription of the HIV-1 RNA genome.Nucleic acids research · 2003Article
- Does the HIV-1 primer activation signal interact with tRNA3(Lys) during the initiation of reverse transcription?Nucleic acids research · 2003Article
- Identification of specific HIV-1 reverse transcriptase contacts to the viral RNA:tRNA complex by mass spectrometry and a primary amine selective reagent.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A 5'-3' long-range interaction in Ty1 RNA controls its reverse transcription and retrotransposition.The EMBO journal · 2002Article
- A Ty1 reverse transcriptase active-site aspartate mutation blocks transposition but not polymerization.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription of the Saccharomyces cerevisiae Ty1 retrotransposon is primed by tRNA(iMet) base paired to the primer binding site (PBS) near the 5' end of Ty1 genomic RNA. The 10-nucleotide PBS is complementary to the last 10 nucleotides of the acceptor stem of tRNA(iMet). A structural probing study of the interactions between the Ty1 RNA template and the tRNA(iMet) primer showed that besides interactions between the PBS and the 3' end of tRNA(iMet), three short regions of Ty1 RNA, named boxes 0, 1, and 2.1, interact with the T and D stems and loops of tRNA(iMet). To determine if these sequences are important for the reverse transcription pathway of the Ty1 retrotransposon, mutant Ty1 elements and tRNA(iMet) were tested for the ability to support transposition. We show that the Ty1 boxes and the complementary sequences in the T and D stems and loops of tRNA(iMet) contain bases that are critical for Ty1 retrotransposition. Disruption of 1 or 2 bp between tRNA(iMet) and box 0, 1, or 2.1 dramatically decreases the level of transposition. Compensatory mutations which restore base pairing between the primer and the template restore transposition. Analysis of the reverse transcription intermediates generated inside Ty1 virus-like particles indicates that initiation of minus-strand strong-stop DNA synthesis is affected by mutations disrupting complementarity between Ty1 RNA and primer tRNA(iMet).
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