ArticleJournal of virology1998
A protein linkage map of the P2 nonstructural proteins of poliovirus.
Article in Journal of virology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- A recurrent adaptive mutation in the transmembrane 2B protein of an insect picorna-like virus in a nonnative host.Journal of virology · 2026Article
- Review
- Crystal structure of a soluble fragment of poliovirus 2CATPase.PLoS pathogens · 2018Article
- A Single Amino Acid Substitution in Poliovirus Nonstructural Protein 2CATPase Causes Conditional Defects in Encapsidation and Uncoating.Journal of virology · 2016Article
- Mapping and modeling of a strain-specific epitope in the Norwalk virus capsid inner shell.Virology · 2016Article
- Picornavirus--host interactions to construct viral secretory membranes.Progress in molecular biology and translational science · 2015Review
- Article
- A C-terminal, cysteine-rich site in poliovirus 2C(ATPase) is required for morphogenesis.The Journal of general virology · 2014Article
- Recombination in enteroviruses is a biphasic replicative process involving the generation of greater-than genome length 'imprecise' intermediates.PLoS pathogens · 2014Article
- Mutations that hamper dimerization of foot-and-mouth disease virus 3A protein are detrimental for infectivity.Journal of virology · 2012Article
- Alanine scanning of poliovirus 2CATPase reveals new genetic evidence that capsid protein/2CATPase interactions are essential for morphogenesis.Journal of virology · 2012Article
- Mechanism of function of viral channel proteins and implications for drug development.International review of cell and molecular biology · 2012Review
- Viral proteins function as ion channels.Biochimica et biophysica acta · 2011Review
- Poliovirus 2C protein forms homo-oligomeric structures required for ATPase activity.The Journal of biological chemistry · 2009Article
- Viral channel-forming proteins.International review of cell and molecular biology · 2009Review
- Functional analysis of picornavirus 2B proteins: effects on calcium homeostasis and intracellular protein trafficking.Journal of virology · 2008Article
- Modification of cellular autophagy protein LC3 by poliovirus.Journal of virology · 2007Article
- Cytopathic mechanisms of HIV-1.Virology journal · 2007Review
- Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase.Virology journal · 2007Article
- Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The yeast two-hybrid system was used to catalog all detectable interactions among the P2 nonstructural cleavage products of poliovirus type 1 (Mahoney). Evidence has been obtained for specific associations among 2A(pro), 2BC, 2C, and 2B. Specifically, 2A(pro) can interact with itself and 2BC and its cleavage products (2B and 2C) interact in all possible combinations, with the exception of 2C/2C. Detected interactions were confirmed in vitro by a glutathione S-transferase pulldown assay, which allowed us to detect 2C/2C association. transdominant-negative mutants of 2B (K. Johnson and P. J. Sarnow, J. Virol. 65:4341-4349, 1991) were examined and were found to retain interaction with wild-type 2B, perhaps reflecting a need for 2B multimerization in viral RNA replication. The multimerization of 2B was examined further by screening a mutagenized library for 2B variants that have lost the ability to bind wild-type 2B. The screen identified two nonconservative missense mutations within a central hydrophobic region, as well as truncations and frameshifts that implicate the C terminus in homointeraction. Introduction of the missense mutations into the genome of the virus conferred a quasi-infectious phenotype, an observation strongly suggesting that the 2B/2B interaction is required for replication of the viral genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.