ArticleNucleic acids research1998
Sp1 activation of a TATA-less promoter requires a species-specific interaction involving transcription factor IID.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 65 citations in OpenAlex.
- The FcRn from gene to protein and function: comparison between species.Frontiers in immunology · 2025Review
- EPA and DHA Enhance CACT Promoter Activity by GABP/NRF2.International journal of molecular sciences · 2024Article
- Genomic targets of the IRE1-XBP1s pathway in mediating metabolic adaptation in epithelial plasticity.Nucleic acids research · 2023Article
- LLGL1 Regulates Gemcitabine Resistance by Modulating the ERK-SP1-OSMR Pathway in Pancreatic Ductal Adenocarcinoma.Cellular and molecular gastroenterology and hepatology · 2020Article
- Discovery, characterization and potential roles of a novel NF-YAx splice variant in human neuroblastoma.Journal of experimental & clinical cancer research : CR · 2019Article
- Regulatory mechanisms leading to differential Acyl-CoA synthetase 4 expression in breast cancer cells.Scientific reports · 2019Article
- SP1 is a transcriptional regulator of URG-4/URGCP gene in hepatocytes.Molecular and cellular biochemistry · 2016Article
- Maximal Expression of the Evolutionarily Conserved Slit2 Gene Promoter Requires Sp1.Cellular and molecular neurobiology · 2016Article
- Sp1-mediated ectopic expression of T-cell lymphoma invasion and metastasis 2 in hepatocellular carcinoma.Cancer medicine · 2016Article
- Gene structure and identification of minimal promoter of Pou2 expressed in spermatogonial cells of rohu carp, Labeo rohita.Molecular biology reports · 2014Article
- cis-Acting elements and trans-acting factors in the transcriptional regulation of raf kinase inhibitory protein expression.PloS one · 2013Article
- Transcriptional regulation of N-acetylglutamate synthase.PloS one · 2012Article
- Molecular characterization of the Schistosoma mansoni zinc finger protein SmZF1 as a transcription factor.PLoS neglected tropical diseases · 2009Article
- Transcriptional dysregulation of TrkA associates with neurodegeneration in spinocerebellar ataxia type 17.Human molecular genetics · 2009Article
- Evolutionary and transcriptional analysis of karyopherin beta superfamily proteins.Molecular & cellular proteomics : MCP · 2008Article
- Constitutive expression of murine decay-accelerating factor 1 is controlled by the transcription factor Sp1.Journal of immunology (Baltimore, Md. : 1950) · 2006Article
- Sp1/3 and NF-1 mediate basal transcription of the human P2X1 gene in megakaryoblastic MEG-01 cells.BMC molecular biology · 2006Article
- oPOSSUM: identification of over-represented transcription factor binding sites in co-expressed genes.Nucleic acids research · 2005Article
- Characterization of the human activator protein-2gamma (AP-2gamma) gene: control of expression by Sp1/Sp3 in breast tumour cells.The Biochemical journal · 2003Article
- Basal transcriptional regulation of human damage-specific DNA-binding protein genes DDB1 and DDB2 by Sp1, E2F, N-myc and NF1 elements.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Sp1 is a ubiquitous activator of numerous TATA-containing and TATA-less promoters within the human genome. This transcription factor is distinct from several other mammalian activators because it cannot stimulate transcription of reporter genes when ectopically expressed in Saccharomyces cerevisiae . Here we report that in cultured cells from Drosophila melanogaster human Sp1 efficiently activates transcription from synthetic promoters containing TATA boxes, but not from promoters that contain an initiator instead of a TATA box. The inability of Sp1 to activate initiator-mediated transcription did not result from inactivity of the consensus initiator element used for the experiments, as other initiator functions were conserved in Drosophila cells. Interestingly, a difference between the Drosophila and human TFIID complexes was found to be responsible for the selective inability of Sp1 to activate initiator-mediated transcription in Drosophila; in a complementation assay with a TFIID-depleted HeLa cell extract both the Drosophila and human TFIID complexes supported TATA-mediated transcription, but only the human complex supported initiator-mediated transcription. These results suggest that a species-specific interaction is required for activation of TATA-less promoters by Sp1, revealing a difference in transcriptional activation mechanisms between vertebrates and invertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.