ArticleNucleic acids research1998
A 7-methylguanosine cap commits U3 and U8 small nuclear RNAs to the nucleolar localization pathway.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 32 citations in OpenAlex.
- An Intermittent Cytochemist.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- Anomalous HIV-1 RNA, How Cap-Methylation Segregates Viral Transcripts by Form and Function.Viruses · 2022Review
- How Cancer Exploits Ribosomal RNA Biogenesis: A Journey beyond the Boundaries of rRNA Transcription.Cells · 2019Review
- Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus.The EMBO journal · 2018Article
- Fibrillarin methylates H2A in RNA polymerase I trans-active promoters in Brassica oleracea.Frontiers in plant science · 2015Article
- Widespread RNA 3'-end oligouridylation in mammals.RNA (New York, N.Y.) · 2012Article
- The nucleus introduced.Cold Spring Harbor perspectives in biology · 2011Review
- The nucleolus.Cold Spring Harbor perspectives in biology · 2011Review
- Apoptosis and autophagy induction in mammalian cells by small interfering RNA knockdown of mRNA capping enzymes.Molecular and cellular biology · 2008Article
- Involvement of nuclear import and export factors in U8 box C/D snoRNP biogenesis.Molecular and cellular biology · 2007Article
- Uncapped mRNA introduced into tobacco protoplasts can be imported into the nucleus and is trapped by leptomycin B.Plant cell reports · 2004Article
- Conserved stem II of the box C/D motif is essential for nucleolar localization and is required, along with the 15.5K protein, for the hierarchical assembly of the box C/D snoRNP.Molecular and cellular biology · 2002Article
- Small nucleolar RNAs: versatile trans-acting molecules of ancient evolutionary origin.Gene expression · 2002Review
- Fluorescent RNA cytochemistry: tracking gene transcripts in living cells.Nucleic acids research · 2001Review
- Prespliceosomal assembly on microinjected precursor mRNA takes place in nuclear speckles.Molecular biology of the cell · 2001Article
- The box C/D motif directs snoRNA 5'-cap hypermethylation.Nucleic acids research · 2000Article
- The nucleolus and the four ribonucleoproteins of translation.The Journal of cell biology · 2000Review
- Genetic and physical interactions involving the yeast nuclear cap-binding complex.Molecular and cellular biology · 1999Article
- Role of the box C/D motif in localization of small nucleolar RNAs to coiled bodies and nucleoli.Molecular biology of the cell · 1999Article
- Nucleolar localization elements of Xenopus laevis U3 small nucleolar RNA.Molecular biology of the cell · 1998Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
U3 and U8 small nucleolar RNAs (snRNAs) participate in pre-rRNA processing. Like the U1, U2, U4 and U5 major spliceosomal snRNAs, U3 and U8 RNAs are transcribed by RNA polymerase II and their initial 7-methylguanosine (m7G) 5' cap structures subsequently become converted to 2,2,7-trimethylguanosine. However, unlike the polymerase II transcribed spliceosomal snRNAs, which are exported to the cytoplasm for cap hypermethylation, U3 and U8 RNAs undergo cap hypermethylation within the nucleus. Human U3 and U8 RNAs with various cap structures were generated by in vitro transcription, fluorescently labeled and microinjected into nuclei of normal rat kidney (NRK) epithelial cells. When U3 and U8 RNAs containing a m7G cap were microinjected they became extensively localized in nucleoli. U3 and U8 RNAs containing alternative cap structures did not localize in nucleoli nor did U3 or U8 RNAs containing triphosphate 5'-termini. The nucleolar localization of m7G-capped U3 RNA was competed by co-microinjection into the nucleus of a 100-fold molar excess of dinucleotide m7GpppG but not by a 100-fold excess of ApppG dinucleotide. Although it was obviously not possible to assess formation of di- and trimethylguanosine caps on the microinjected U3 and U8 RNAs in these single cell experiments, these results indicate that the initial presence of a m7G cap on U3 and U8 RNAs, most likely together with internal sequence elements, commits these transcripts to the nucleolar localization pathway and point to diverse roles of the m7G cap in the intracellular traffic of various RNAs transcribed by RNA polymerase II.
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