ArticleNucleic acids research1998
Determination of interactions between structured nucleic acids by fluorescence resonance energy transfer (FRET): selection of target sites for functional nucleic acids.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 43 citations in OpenAlex.
- Phase-specific RNA accumulation and duplex thermodynamics in multiphase coacervate models for membraneless organelles.Nature chemistry · 2022Article
- Insight into the biochemical mechanism of DNA helicases provided by bulk-phase and single-molecule assays.Methods (San Diego, Calif.) · 2022Review
- A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence ofRSC advances · 2022Article
- Real-time fluorescent image analysis of DNA spot hybridization kinetics to assess microarray spot heterogeneity.Analytical chemistry · 2012Article
- New concepts of fluorescent probes for specific detection of DNA sequences: bis-modified oligonucleotides in excimer and exciplex detection.The Libyan journal of medicine · 2009Article
- Real-time DNA microarray analysis.Nucleic acids research · 2009Article
- Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Effects of metallic silver particles on resonance energy transfer in labeled bovine serum albumin.Biochemical and biophysical research communications · 2002Article
- Enhanced Emission Induced by FRET from a Long-Lifetime, Low Quantum Yield Donor to a Long-Wavelength, High Quantum Yield Acceptor.Journal of fluorescence · 2002Article
- On the possibility of long-wavelength long-lifetime high-quantum-yield luminophores.Analytical biochemistry · 2001Article
- Double-labeled donor probe can enhance the signal of fluorescence resonance energy transfer (FRET) in detection of nucleic acid hybridization.Nucleic acids research · 2000Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously developed a method for monitoring the integrity of oligonucleotides in vitro and in vivo by quantitating fluorescence resonance energy transfer (FRET) between two different fluorochromes attached to a single oligonucleotide. As an extension of this analysis, we examined changes in the extent of FRET in the presence or absence of target nucleic acids with a specific sequence and a higher-ordered structure. In this system FRET was maximal when probes were free in solution and a decrease in FRET was evidence of successful hybridization. We used a single-stranded oligodeoxyribonucleotide labeled at its 5'-end and its 3'-end with 6-carboxyfluorescein and 6-carboxytetramethylrhodamine, respectively. Incubation of the probe with a single-stranded complementary oligonucleotide reduced the FRET. Moreover, a small change in FRET was also observed when the probe was incubated with an oligonucleotide in which the target site had been embedded in a stable hairpin structure. The decrease in the extent of FRET depended on the length of the stem region of the hairpin structure and also on the higher-ordered structure of the probe. These results indicate that this spectrofluorometric method and FRET probes can be used to estimate the efficacy of hybridization between a probe and its target site within highly ordered structures. This conclusion based on changes in FRET was confirmed by gel-shift assays.
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