ArticleNucleic acids research1998
The role of zinc finger linkers in p43 and TFIIIA binding to 5S rRNA and DNA.
Article in Nucleic acids research, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 39 citations in OpenAlex.
- The Essential Role of Prolines and Their Conformation in Allosteric Regulation of Kaiso Zinc Finger DNA-Binding Activity by the Adjacent C-Terminal Loop.International journal of molecular sciences · 2022Article
- The role of zinc finger linkers in zinc finger protein binding to DNA.Journal of computer-aided molecular design · 2021Article
- Improving selectivity of DNA-RNA binding zinc finger using directed evolution.BMC research notes · 2019Article
- Genetic Variation in Human Gene Regulatory Factors Uncovers Regulatory Roles in Local Adaptation and Disease.Genome biology and evolution · 2019Article
- An RNA aptamer to HP1/Swi6 facilitates heterochromatin formation at an ectopic locus in S.pombe.RNA biology · 2019Article
- Role of protein structure and the role of individual fingers in zinc finger protein-DNA recognition: a molecular dynamics simulation study and free energy calculations.Journal of computer-aided molecular design · 2018Article
- Structural Insights into c-Myc-interacting Zinc Finger Protein-1 (Miz-1) Delineate Domains Required for DNA Scanning and Sequence-specific Binding.The Journal of biological chemistry · 2017Article
- The functional significance of common polymorphisms in zinc finger transcription factors.G3 (Bethesda, Md.) · 2014Article
- The natural history of the WRKY-GCM1 zinc fingers and the relationship between transcription factors and transposons.Nucleic acids research · 2006Article
- Reduction in DNA-binding affinity of Cys2His2 zinc finger proteins by linker phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- CTCF As an Example of DNA-Binding Transcription Factors Containing Clusters of C2H2-Type Zinc Fingers.Acta naturaeArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct complexes with 5S ribosomal RNA in Xenopus oocytes. Additionally, TFIIIA binds the internal promoter of the 5S RNA gene and supports assembly of a transcription initiation complex. Both proteins have nine tandemly repeated zinc fingers with almost identical linker lengths between corresponding fingers, yet p43 has no detectable affinity for the 5S RNA gene. TFIIIA zinc fingers 1-3 are connected by highly conserved linkers, first identified in the Drosophila protein Krüppel, that are found in many DNA binding zinc finger proteins. To understand the role of these linkers in RNA and DNA binding we exchanged three TFIIIA linker amino acids with the equivalent amino acids from p43. The major effect of linker substitution is a 50-fold reduction in DNA specificity, concomitant with an 8-fold reduction in affinity. N-Terminal zinc fingers from either TFIIIA or p43 bind to multiple specific sites on 5S RNA that are resistant to competition by tRNA or poly(rA). This mode of RNA binding is unaffected by linker substitution. These data suggest that zinc finger linkers significantly facilitate the specificity of DNA binding.
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