ArticleThe Journal of cell biology1998
Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 162 papers.
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Who cites it
162 citing papers in PubMed, 371 citations in OpenAlex.
- Article
- Bni5 regulates and coordinates septin architecture and myosin-II functions at the cell division site.The Journal of cell biology · 2025Article
- Article
- Central Role of the Actomyosin Ring in Coordinating Cytokinesis Steps in Budding Yeast.Journal of fungi (Basel, Switzerland) · 2024Review
- Septin Organization and Dynamics for Budding Yeast Cytokinesis.Journal of fungi (Basel, Switzerland) · 2024Review
- Improved tools for live imaging of F-actin structures in yeast.Molecular biology of the cell · 2024Article
- Phosphorylation of the F-BAR protein Hof1 drives septin ring splitting in budding yeast.Nature communications · 2024Article
- Unraveling the mechanisms and evolution of a two-domain module in IQGAP proteins for controlling eukaryotic cytokinesis.Cell reports · 2023Article
- Septins regulate border cell surface geometry, shape, and motility downstream of Rho in Drosophila.Developmental cell · 2023Article
- Characterization of cell-to-cell variation in nuclear transport rates and identification of its sources.iScience · 2023Article
- PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis.Cellular and molecular life sciences : CMLS · 2022Article
- Article
- The Saccharomyces cerevisiae Ncw2 protein works on the chitin/β-glucan organisation of the cell wall.Antonie van Leeuwenhoek · 2021Article
- Cellular functions of actin- and microtubule-associated septins.Current biology : CB · 2021Review
- Article
- Septin Assembly and Remodeling at the Cell Division Site During the Cell Cycle.Frontiers in cell and developmental biology · 2021Review
- The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems.Frontiers in cellular and infection microbiology · 2021Review
- Cleavage-furrow formation without F-actin inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Comparative Analysis of the Roles of Non-muscle Myosin-IIs in Cytokinesis in Budding Yeast, Fission Yeast, and Mammalian Cells.Frontiers in cell and developmental biology · 2020Review
102 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a Saccharomyces cerevisiae protein, Cyk1p, that exhibits sequence similarity to the mammalian IQGAPs. Gene disruption of Cyk1p results in a failure in cytokinesis without affecting other events in the cell cycle. Cyk1p is diffused throughout most of the cell cycle but localizes to a ring structure at the mother-bud junction after the initiation of anaphase. This ring contains filamentous actin and Myo1p, a myosin II homologue. In vivo observation with green fluorescent protein-tagged Myo1p showed that the ring decreases drastically in size during cell division and therefore may be contractile. These results indicate that cytokinesis in budding yeast is likely to involve an actomyosin-based contractile ring. The assembly of this ring occurs in temporally distinct steps: Myo1p localizes to a ring that overlaps the septins at the G1-S transition slightly before bud emergence; Cyk1p and actin then accumulate in this ring after the activation of the Cdc15 pathway late in mitosis. The localization of myosin is abolished by a mutation in Cdc12p, implicating a role for the septin filaments in the assembly of the actomyosin ring. The accumulation of actin in the cytokinetic ring was not observed in cells depleted of Cyk1p, suggesting that Cyk1p plays a role in the recruitment of actin filaments, perhaps through a filament-binding activity similar to that demonstrated for mammalian IQGAPs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.