ArticleThe Plant cell1997
Biochemical characterization of Arabidopsis wild-type and mutant phytochrome B holoproteins.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Integrating protein sequence design and evolutionary sequence conservation to uncover spectral tuning sites in red-light photoreceptors.Structure (London, England : 1993) · 2025Article
- SUPPRESSOR OF PHYTOCHROME B-4 #3 reduces the expression of PIF-activated genes and increases expression of growth repressors to regulate hypocotyl elongation in short days.BMC plant biology · 2022Article
- Bifunctional regulators of photoperiodic flowering in short day plant rice.Frontiers in plant science · 2022Review
- Differing biophysical properties underpin the unique signaling potentials within the plant phytochrome photoreceptor families.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Carotenoid Biosynthesis and Plastid Development in Plants: The Role of Light.International journal of molecular sciences · 2021Review
- PCH1 regulates light, temperature, and circadian signaling as a structural component of phytochrome B-photobodies inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- PCH1 and PCHL promote photomorphogenesis in plants by controlling phytochrome B dark reversion.Nature communications · 2017Article
- Photosensing and Thermosensing by Phytochrome B Require Both Proximal and Distal Allosteric Features within the Dimeric Photoreceptor.Scientific reports · 2017Article
- Phytochrome B Nuclear Bodies Respond to the Low Red to Far-Red Ratio and to the Reduced Irradiance of Canopy Shade in Arabidopsis.Plant physiology · 2014Article
- Photobody Localization of Phytochrome B Is Tightly Correlated with Prolonged and Light-Dependent Inhibition of Hypocotyl Elongation in the Dark.Plant physiology · 2014Article
- Gene duplication and the environmental regulation of physiology and development.Ecology and evolution · 2014Article
- Article
- Structure-guided engineering of plant phytochrome B with altered photochemistry and light signaling.Plant physiology · 2013Article
- Phosphorylation of phytochrome B inhibits light-induced signaling via accelerated dark reversion in Arabidopsis.The Plant cell · 2013Article
- Shade avoidance.The arabidopsis book · 2012Article
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- Article
- Residues clustered in the light-sensing knot of phytochrome B are necessary for conformer-specific binding to signaling partner PIF3.PLoS genetics · 2009Article
- Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Mutant screen distinguishes between residues necessary for light-signal perception and signal transfer by phytochrome B.PLoS genetics · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Although phytochrome B (phyB) plays a particularly important role throughout the life cycle of a plant, it has not been studied in detail at the molecular level due to its low abundance. Here, we report on the expression, assembly with chromophore, and purification of epitope-tagged Arabidopsis phyB. In addition, we have reconstructed two missense mutations, phyB-4 and phyB-101, isolated in long hypocotyl screens. We show that mutant proteins phyB-4 and phyB-101 exhibit altered spectrophotometric and biochemical properties relative to the wild-type protein. In particular, we demonstrate that phyB-101 Pfr exhibits rapid nonphotochemical (dark) reversion to Pr that results in a lower photoequilibrium level of the active Pfr form. We conclude that this occurs in vivo as well because phyB-101 mutants are shown to lack an end-of-day-far-red hypocotyl elongation response that requires a stable Pfr species. We propose that this Pfr instability may be the primary molecular mechanism underlying the phyB-101 mutant phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.