ArticleThe Plant cell1997
Stable transformation of an Arabidopsis cell suspension culture with firefly luciferase providing a cellular system for analysis of chaperone activity in vivo.
Article in The Plant cell, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 117 citations in OpenAlex.
- Denaturation of firefly luciferase at heat shock temperatures captured in silico.Biophysical journal · 2025Article
- Epigenetic regulation of lignin biosynthesis in wood formation.The New phytologist · 2025Article
- From the archives: Meristem transitions in wheat spike development, detox of reactive carbonyl species in plant metabolism, and transformation of Arabidopsis cells.The Plant cell · 2022Article
- CRISPR screens in plants: approaches, guidelines, and future prospects.The Plant cell · 2021Review
- Seasonal Growth ofPlants (Basel, Switzerland) · 2018Article
- Solar irradiation levels during simulated long- and short-term heat waves significantly influence heat survival, pigment and ascorbate composition, and free radical scavenging activity in alpine Vaccinium gaultherioides.Physiologia plantarum · 2018Article
- Article
- Class I and II Small Heat Shock Proteins Together with HSP101 Protect Protein Translation Factors during Heat Stress.Plant physiology · 2016Article
- Ectopic expression of GroEL from Xenorhabdus nematophila in tomato enhances resistance against Helicoverpa armigera and salt and thermal stress.Transgenic research · 2015Article
- Expression analysis of nine small heat shock protein genes from Tamarix hispida in response to different abiotic stresses and abscisic acid treatment.Molecular biology reports · 2014Article
- Recovery from heat, salt and osmotic stress in Physcomitrella patens requires a functional small heat shock protein PpHsp16.4.BMC plant biology · 2013Article
- Development of a high-throughput screening cancer cell-based luciferase refolding assay for identifying Hsp90 inhibitors.Assay and drug development technologies · 2013Article
- Article
- 40S ribosome biogenesis co-factors are essential for gametophyte and embryo development.PloS one · 2013Article
- Approaches to engineer stability of beetle luciferases.Computational and structural biotechnology journal · 2012Review
- Two ancient bacterial-like PPP family phosphatases from Arabidopsis are highly conserved plant proteins that possess unique properties.Plant physiology · 2011Article
- VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system.Plant physiology · 2010Article
- The molecular basis for stress-induced acquisition of somatic embryogenesis.Molecular biology reports · 2010Review
- The heat shock response in moss plants is regulated by specific calcium-permeable channels in the plasma membrane.The Plant cell · 2009Article
- Arabidopsis microtubule-associated protein AtMAP65-2 acts as a microtubule stabilizer.Plant molecular biology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using Agrobacterium, we developed a method to transform an Arabidopsis cell suspension culture. A stably transformed cell line expressing high levels of firefly luciferase (Luc) was used for in vivo studies of thermal denaturation and renaturation of the enzyme and the protective role of different chaperones. Luc activity was monitored under heat stress and recovery conditions in control, thermotolerant cells and cells expressing plant chaperones after transient cotransformation with plasmids encoding proteins of the heat shock protein Hsp90, Hsp70, or Hsp20 family. The effects of the expressed proteins were specific. The Hsp17.6 class I protein maintained Luc activity on a level comparable with that observed in thermotolerant cells and improved Luc renaturation. Although transient expression of Hsp90 did not protect Luc from thermal denaturation, it accelerated Luc renaturation during recovery. In contrast to the other chaperones tested, overexpression of Hsp70 alone had no effect on denaturation and renaturation of Luc but enhanced Luc renaturation if coexpressed with Hsp17.6.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.