ArticleThe Journal of clinical investigation1998
Bone morphogenetic protein-6 production in human osteoblastic cell lines. Selective regulation by estrogen.
Article in The Journal of clinical investigation, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 93 citations in OpenAlex.
- Postmenopausal osteoporosis and vascular calcification: The estrogen regulation network and calcification paradox.Journal of translational internal medicine · 2026Article
- Cytochrome P450 1B1 Expression Regulates Intracellular Iron Levels and Oxidative Stress in the Retinal Endothelium.International journal of molecular sciences · 2023Article
- Skeletal Effects of Inducible ERα Deletion in Osteocytes in Adult Mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- The Induction of Bone Formation: The Translation Enigma.Frontiers in bioengineering and biotechnology · 2018Review
- The effect of magnesium on early osseointegration in osteoporotic bone: a histological and gene expression investigation.Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA · 2017Article
- Hypermethylation leads to bone morphogenetic protein 6 downregulation in hepatocellular carcinoma.PloS one · 2014Article
- Expression of bone morphogenetic protein-6 in dental follicle stem cells and its effect on osteogenic differentiation.Cells, tissues, organs · 2013Article
- BRITER: a BMP responsive osteoblast reporter cell line.PloS one · 2012Article
- Direct transcriptional targets of sex steroid hormones in bone.Journal of cellular biochemistry · 2011Review
- Unique ERalpha cistromes control cell type-specific gene regulation.Molecular endocrinology (Baltimore, Md.) · 2008Article
- Expression and synthesis of bone morphogenetic proteins by osteoclasts: a possible path to anabolic bone remodeling.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Expression of genes for bone morphogenetic proteins BMP-2, BMP-4 and BMP-6 in various parts of the human skeleton.BMC musculoskeletal disorders · 2007Article
- Hormones and bone health in postmenopausal women.Endocrine · 2004Review
- Distribution of estrogen receptors alpha and beta mRNA in mouse urogenital tissues and their expression after oophorectomy and estrogen replacement.International urogynecology journal and pelvic floor dysfunction · 2003Article
- A murine osteosarcoma cell line with a potential to develop ossification upon transplantation.Japanese journal of cancer research : Gann · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bone morphogenetic proteins (BMPs) induce differentiation of osteoblast and chondroblast lineage cells from uncommitted mesenchymal precursors. Because estrogen has potent osteochondrogenic actions, we investigated its effect on BMP production in two estrogen-responsive, human immortalized cell lines (hFOB/ER3 and hFOB/ER9) that display the mature osteoblast phenotype. These cell lines were produced by stable transfection of the estrogen receptor (ER) gene into immortalized fetal osteoblasts at low ( approximately 800 ER/ nucleus) and at high ( approximately 3, 900 ER/nucleus) levels, respectively. As assessed by reverse transcriptase PCR, treatment with 17beta-estradiol (10(-)10 - 10(-)7 M) increased steady-state levels of BMP-6 mRNA dose dependently by twofold in the hFOB/ER3 cells and by over threefold in the hFOB/ER9 cells. Messenger RNA levels for transforming growth factors-beta1 and -beta2 and BMPs-1 through -5 and -7 levels were unchanged. The results were confirmed by sequence determination of the PCR product and by Northern blot analysis for total RNA. 17beta-estradiol increased BMP-6 protein production sixfold by Western analysis. Cotreatment with antiestrogens (ICI 182,780 or 4-hydroxytamoxifen) antagonized the effects of 17beta-estradiol. These data suggest that some of the skeletal effects of estrogen on bone and cartilage may be mediated by increased production of BMP-6 by osteoblasts.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.