ArticleProceedings of the National Academy of Sciences of the United States of America1998
5-Lipoxygenase expression in Langerhans cells of normal human epidermis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 64 citations in OpenAlex.
- RNAi-based ALOX15B silencing augments keratinocyte inflammation in vitro via EGFR/STAT1/JAK1 signalling.Cell death & disease · 2025Article
- Metabolic pathways of eicosanoids-derivatives of arachidonic acid and their significance in skin.Cellular & molecular biology letters · 2025Review
- The role of human 5-Lipoxygenase (5-LO) in carcinogenesis - a question of canonical and non-canonical functions.Oncogene · 2024Review
- Synthesis,Journal of enzyme inhibition and medicinal chemistry · 2023Article
- Iron-Dependent Trafficking of 5-Lipoxygenase and Impact on Human Macrophage Activation.Frontiers in immunology · 2019Article
- Protective role of 5-lipoxigenase during Leishmania infantum infection is associated with Th17 subset.BioMed research international · 2014Article
- High expression of 5-lipoxygenase in normal and malignant mantle zone B lymphocytes.BMC immunology · 2009Article
- Expression of 5-lipoxygenase (5-LOX) in T lymphocytes.Immunology · 2007Article
- Leukotrienes modulate cytokine release from dendritic cells.Immunology · 2005Article
- 5-Lipoxygenase Pathway, Dendritic Cells, and Adaptive Immunity.Journal of biomedicine & biotechnology · 2004Article
- Expanding expression of the 5-lipoxygenase pathway within the arterial wall during human atherogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- IL-4 determines eicosanoid formation in dendritic cells by down-regulation of 5-lipoxygenase and up-regulation of 15-lipoxygenase 1 expression.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
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Authors and funding
12 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied expression of the 5-lipoxygenase (5-LO) pathway in normal human skin. In situ hybridization revealed a 5-LO mRNA-containing epidermal cell (EC) population that was predominantly located in the midportion of the spinous layer, in outer hair root sheaths, and in the epithelial compartment of sebaceous glands. Examination of skin specimens by immunohistochemistry and of primary ECs by flow cytometry mapped the 5-LO protein exclusively to Langerhans cells (LCs). The LC 5-LO protein was largely found in the nuclear matrix, in nuclear envelopes, and perinuclear regions as indicated by in situ confocal laser scan microscopy. Reverse transcription-PCR and immunoblot analyses of purified primary EC populations further indicated that LCs are major 5-LO expressing cells. Enriched primary LCs were also found to contain 5-LO activating protein (FLAP), leukotriene (LT) C4 synthase, and LTA4 hydrolase. By contrast, 5-LO, FLAP, and LTC4 synthase were undetectable or largely reduced, but LTA4 hydrolase transcripts and protein were identified in ECs depleted of LCs. These data show that naive LCs are major, and possibly the sole, 5-LO pathway expressing cells in the normal human epidermis.
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