ArticleProceedings of the National Academy of Sciences of the United States of America1998
Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Chi hotspot Control of RecBCD Helicase-nuclease: Enzymatic Tests Support the Intramolecular Signal-transduction Model.Journal of molecular biology · 2024Article
- The Streptococcus agalactiae Exonuclease ExoVII Is Required for Resistance to Exogenous DNA-Damaging Agents.Journal of bacteriology · 2023Article
- RecBCD enzyme and Chi recombination hotspots as determinants of self vs. non-self: Myths and mechanisms.Advances in genetics · 2022Article
- Adaptive Laboratory Evolution as a Means To Generate Lactococcus lactis Strains with Improved Thermotolerance and Ability To Autolyze.Applied and environmental microbiology · 2021Article
- Characterization of a Prophage-Free Derivative Strain ofFrontiers in microbiology · 2018Article
- Unexpected DNA context-dependence identifies a new determinant of Chi recombination hotspots.Nucleic acids research · 2016Article
- λ Recombination and Recombineering.EcoSal Plus · 2016Review
- End-resection at DNA double-strand breaks in the three domains of life.Biochemical Society transactions · 2013Review
- Co-evolution of segregation guide DNA motifs and the FtsK translocase in bacteria: identification of the atypical Lactococcus lactis KOPS motif.Nucleic acids research · 2012Article
- Molecular determinants responsible for recognition of the single-stranded DNA regulatory sequence, χ, by RecBCD enzyme.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain.Nucleic acids research · 2011Article
- DNA motifs that sculpt the bacterial chromosome.Nature reviews. Microbiology · 2011Review
- Two coregulated efflux transporters modulate intracellular heme and protoporphyrin IX availability in Streptococcus agalactiae.PLoS pathogens · 2010Article
- Multiplicity of DNA end resection machineries in chromosome break repair.Genes & development · 2009Article
- RecBCD enzyme and the repair of double-stranded DNA breaks.Microbiology and molecular biology reviews : MMBR · 2008Review
- Identification of DNA motifs implicated in maintenance of bacterial core genomes by predictive modeling.PLoS genetics · 2007Article
- Genomic plasticity of the rrn-nqrF intergenic segment in the Chlamydiaceae.Journal of bacteriology · 2007Article
- Natural meiotic recombination hot spots in the Schizosaccharomyces pombe genome successfully predicted from the simple sequence motif M26.Molecular and cellular biology · 2005Article
- Comparative and evolutionary analysis of the bacterial homologous recombination systems.PLoS genetics · 2005Article
- The recombination genes addAB are not restricted to gram-positive bacteria: genetic analysis of the recombination initiation enzymes RecF and AddAB in Rhizobium etli.Journal of bacteriology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Studies of RecBCD-Chi interactions in Escherichia coli have served as a model to understand recombination events in bacteria. However, the existence of similar interactions has not been demonstrated in bacteria unrelated to E. coli. We developed an in vivo model to examine components of dsDNA break repair in various microorganisms. Here, we identify the major exonuclease in Lactococcus lactis, a Gram-positive organism evolutionarily distant from E. coli, and provide evidence for exonuclease-Chi interactions. Insertional mutants of L. lactis, screened as exonuclease-deficient, affected a single locus and resulted in UV sensitivity and recombination deficiency. The cloned lactococcal genes (called rexAB) restored UV resistance, recombination proficiency, and the capacity to degrade linear DNA, to an E. coli recBCD mutant. In this context, DNA degradation is specifically blocked by the putative lactococcal Chi site (5'-GCGCGTG-3'), but not by the E. coli Chi (5'-GCTGGTGG-3') site. RexAB-mediated recombination was shown to be stimulated approximately 27-fold by lactococcal Chi. Our results reveal that RexAB fulfills the biological roles of RecBCD and indicate that its activity is modulated by a short DNA sequence. We speculate that exonuclease/recombinase enzymes whose activities are modulated by short DNA sequences are widespread among bacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.