ArticleProceedings of the National Academy of Sciences of the United States of America1998
Molecular cloning of a human cDNA encoding beta-1,4-galactosyltransferase with 37% identity to mammalian UDP-Gal:GlcNAc beta-1,4-galactosyltransferase.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 123 citations in OpenAlex.
- Central Roles of Glucosylceramide in Driving Cancer Pathogenesis.International journal of molecular sciences · 2025Review
- Therapeutic effect of galactosyltransferase- and sialyltransferase-encoding mRNA in rheumatoid arthritis.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Glycosphingolipids: from metabolism to chemoenzymatic total synthesis.Organic & biomolecular chemistry · 2024Review
- Decreased B4GALT1 promotes hepatocellular carcinoma cell invasiveness by regulating the laminin-integrin pathway.Oncogenesis · 2023Article
- Challenge to the suppression of tumor growth by the β4-galactosyltransferase genes.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2015Review
- Biosynthetic Machinery Involved in Aberrant Glycosylation: Promising Targets for Developing of Drugs Against Cancer.Frontiers in oncology · 2015Review
- Deciphering the glycogenome of schistosomes.Frontiers in genetics · 2014Review
- Involvement of murine β-1,4-galactosyltransferase V in lactosylceramide biosynthesis.Glycoconjugate journal · 2010Article
- The expression patterns of beta1,4 galactosyltransferase I and V mRNAs, and Galbeta1-4GlcNAc group in rat gastrocnemius muscles post sciatic nerve injury.Glycoconjugate journal · 2008Article
- N-Glycan fucosylation of epidermal growth factor receptor modulates receptor activity and sensitivity to epidermal growth factor receptor tyrosine kinase inhibitor.Cancer science · 2008Article
- Protein N-glycosylation in the baculovirus-insect cell system.Current drug targets · 2007Review
- The role of TNF-alpha and its receptors in the production of beta-1,4 galactosyltransferase I and V mRNAs by rat primary astrocytes.Journal of molecular neuroscience : MN · 2007Article
- Molecular cloning and functional characterization of a Lepidopteran insect beta4-N-acetylgalactosaminyltransferase with broad substrate specificity, a functional role in glycoprotein biosynthesis, and a potential functional role in glycolipid biosynthesis.The Journal of biological chemistry · 2004Article
- Distinct patterns of expression of the beta-1,4-galactosyltransferases during testicular development in the mouse.Molecular and cellular biochemistry · 2003Article
- Identification and characterization of a Drosophila melanogaster ortholog of human beta1,4-galactosyltransferase VII.Glycobiology · 2002Article
- Human airway mucin glycosylation: a combinatory of carbohydrate determinants which vary in cystic fibrosis.Glycoconjugate journal · 2001Review
- Knockout of mouse beta 1,4-galactosyltransferase-1 gene results in a dramatic shift of outer chain moieties of N-glycans from type 2 to type 1 chains in hepatic membrane and plasma glycoproteins.The Biochemical journal · 2001Article
- UDP-N-Acetyl-alpha-D-glucosamine as acceptor substrate of beta-1,4-galactosyltransferase. Enzymatic synthesis of UDP-N-acetyllactosamine.Glycoconjugate journal · 1999Article
- Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Differences in N-acetyllactosamine synthesis between beta-1,4-galactosyltransferases I and V.Glycoconjugate journal · 1999Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a beta-1,4-galactosyltransferase named beta-1,4-GalT II was cloned from a cDNA library of the human breast tumor cell line, MRK-nu-1. Initially, a 860-bp PCR fragment was obtained from MRK-nu-1 mRNA by 3'-rapid amplification of cDNA ends by using two nested degenerate oligonucleotide primers based on a highly conserved amino acid sequence found in the catalytic domain of mammalian beta-1,4-galactosyltransferases and Lymnaea stagnalis beta-1,4-N-acetylglucosaminyltransferase (beta-1,4-GlcNAcT), both of which utilize the same sugar acceptor. This subsequently was used as a probe to isolate a 4.7-kb cDNA that contained an ORF of 1,164 bp predicting a polypeptide of 388 aa. Its deduced amino acid sequence shows an identity of 37% with that of the previously characterized human beta-1,4-galactosyltransferase (referred to as beta-1,4-GalT I) and of 28% with that of L. stagnalis beta-1,4-GlcNAcT. Study of the properties of the beta-1,4-GalT II fused to protein A expressed as a soluble form in COS-7 cells revealed that it is a genuine beta-1,4-GalT but has no lactose synthetase activity in the presence of alpha-lactalbumin. Northern blot analysis of 24 human tissues showed that they all express the beta-1,4-GalT II transcript, although the levels varied. These results indicate that human cells contain another beta-1,4-GalT.
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Registered trials
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