ArticleJournal of clinical microbiology1998
Comparative analysis of infrequent-restriction-site PCR and pulsed-field gel electrophoresis for epidemiological typing of Legionella pneumophila serogroup 1 strains.
Article in Journal of clinical microbiology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 55 citations in OpenAlex.
- Complete Genome Sequences of Legionella pneumophila subsp. fraseri Strains Detroit-1 and Dallas 1E.Genome announcements · 2017Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- Genomic diversity of Legionella pneumophila serogroup 1 from environmental water sources and clinical specimens using pulsed-field gel electrophoresis (PFGE) from 1985 to 2007, Korea.Journal of microbiology (Seoul, Korea) · 2010Article
- Clinical and environmental isolates of Legionella pneumophila serogroup 1 cannot be distinguished by sequence analysis of two surface protein genes and three housekeeping genes.Applied and environmental microbiology · 2005Article
- DNA fingerprinting of Ralstonia paucula by infrequent-restriction-site PCR and randomly amplified polymorphic DNA analysis.Journal of clinical microbiology · 2003Article
- Characterization of members of the Legionellaceae family by automated ribotyping.Journal of clinical microbiology · 2003Article
- Legionella and Legionnaires' disease: 25 years of investigation.Clinical microbiology reviews · 2002Review
- Characterization of Listeria monocytogenes strains involved in invasive and noninvasive listeriosis outbreaks by PCR-based fingerprinting techniques.Applied and environmental microbiology · 2001Article
- Suitability of PCR fingerprinting, infrequent-restriction-site PCR, and pulsed-field gel electrophoresis, combined with computerized gel analysis, in library typing of Salmonella enterica serovar enteritidis.Applied and environmental microbiology · 2000Article
- Isolation of Legionella oakridgensis from two patients with pleural effusion living in the same geographical area.Journal of clinical microbiology · 2000Article
- Differentiation of pathogenic Bartonella species by infrequent restriction site PCR.Journal of clinical microbiology · 2000Article
- Application of infrequent-restriction-site PCR to clinical isolates of Acinetobacter baumannii and Serratia marcescens.Journal of clinical microbiology · 1999Article
- Australian isolates of Legionella longbeachae are not a clonal population.Journal of clinical microbiology · 1999Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two methods were compared for the analysis of 48 unrelated and epidemiologically related Legionella pneumophila serogroup 1 isolates. These are the infrequent-restriction-site PCR (IRS-PCR) assay with adapters designed for XbaI and PstI restriction sites and the pulsed-field gel electrophoresis (PFGE) analysis determined after DNA restriction with SfiI. Both methods demonstrated a high level of discrimination with a similar capacity for differentiating 23 of the 24 unrelated isolates. PFGE analysis and IRS-PCR assay were both able to identify epidemiologically related isolates of L. pneumophila from three outbreaks. Hence, IRS-PCR assay appears to be a reproducible (intergel reproducibility, 100%) and discriminative (discriminatory index, > or = 0.996) tool for typing of Legionella. Compared to PFGE, however, IRS-PCR presented an advantage through ease of performance and with attributes of rapidity and sensitivity of target DNA.
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Registered trials
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