ArticleThe EMBO journal1998
Regulation of the G1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 112 citations in OpenAlex.
- CDK phosphorylation of Sfr1 downregulates Rad51 function in late-meiotic homolog invasions.The EMBO journal · 2024Article
- Phosphorylation of Schizosaccharomyces pombe Dss1 mediates direct binding to the ubiquitin-ligase Dma1 in vitro.Protein science : a publication of the Protein Society · 2023Article
- The ecl family gene ecl3+ is induced by phosphate starvation and contributes to sexual differentiation in fission yeast.Journal of cell science · 2023Article
- The Meiosis-Specific Crs1 Cyclin Is Required for Efficient S-Phase Progression and Stable Nuclear Architecture.International journal of molecular sciences · 2021Article
- Computational modeling of chromosome re-replication in mutant strains of fission yeast.Molecular biology of the cell · 2021Article
- Requirement of PP2A-B56iScience · 2020Article
- Protein Phosphatases in G1 Regulation.International journal of molecular sciences · 2020Review
- Down-regulation of Cdk1 activity in G1 coordinates the G1/S gene expression programme with genome replication.Current genetics · 2019Review
- CDK contribution to DSB formation and recombination in fission yeast meiosis.PLoS genetics · 2019Article
- The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi.Frontiers in cellular and infection microbiology · 2019Review
- Adding phosphorylation events to the core oscillator driving the cell cycle of fission yeast.PloS one · 2018Article
- Cell cycle control by a minimal Cdk network.PLoS computational biology · 2015Article
- Molecular mechanisms creating bistable switches at cell cycle transitions.Open biology · 2013Article
- The fission yeast minichromosome maintenance (MCM)-binding protein (MCM-BP), Mcb1, regulates MCM function during prereplicative complex formation in DNA replication.The Journal of biological chemistry · 2013Article
- The role of the RACK1 ortholog Cpc2p in modulating pheromone-induced cell cycle arrest in fission yeast.PloS one · 2013Article
- Induction of a G1-S checkpoint in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mathematical modeling of fission yeast Schizosaccharomyces pombe cell cycle: exploring the role of multiple phosphatases.Systems and synthetic biology · 2011Article
- Article
- Multisite phosphorylation provides an effective and flexible mechanism for switch-like protein degradation.PloS one · 2010Article
- Ubiquitylation and proteasomal degradation of the p21(Cip1), p27(Kip1) and p57(Kip2) CDK inhibitors.Cell cycle (Georgetown, Tex.) · 2010Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In fission yeast, the cyclin-dependent kinase (CDK) inhibitor p25(rum1) is a key regulator of progression through the G1 phase of the cell cycle. We show here that p25(rum1) protein levels are sharply periodic. p25(rum1) begins to accumulate at anaphase, persists in G1 and is destroyed during S phase. p25(rum1 )is stabilized and polyubiquitinated in a mutant defective in the 26S proteasome, suggesting that its degradation normally occurs through the ubiquitin-dependent 26S proteasome pathway. Phosphorylation of p25(rum1 )by cdc2-cyclin complexes at residues T58 and T62 is important to target the protein for degradation. Mutation of one or both of these residues to alanine causes stabilization of p25(rum1) and induces a cell cycle delay in G1 and polyploidization due to occasional re-initiation of DNA replication before mitosis. The CDK-cyclin complex cdc2-cig1, which is insensitive to p25(rum1 )inhibition, seems to be the main kinase that phosphorylates p25(rum1). Phosphorylation of p25(rum1) in S phase and G2 serves as the trigger for p25(rum1) proteolysis. Thus, periodic accumulation and degradation of the CDK inhibitor p25(rum1 )in G1 plays a role in setting a threshold of cyclin levels important in determining the length of the pre-Start G1 phase and in ensuring the correct order of cell cycle events.
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