ArticleThe EMBO journal1998
The heterotrimeric G protein Go2 regulates catecholamine uptake by secretory vesicles.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 63 citations in OpenAlex.
- Targeting GαBasic research in cardiology · 2024Article
- Packaging monoamine neurotransmitters.Cell research · 2024Article
- Vesicular neurotransmitter transporters in Drosophila melanogaster.Biochimica et biophysica acta. Biomembranes · 2020Review
- Striatal dopamine neurotransmission: regulation of release and uptake.Basal ganglia · 2016Article
- N-terminus regulation of VMAT2 mediates methamphetamine-stimulated efflux.Neuroscience · 2014Article
- The α-subunit of the trimeric GTPase Go2 regulates axonal growth.Journal of neurochemistry · 2013Article
- Article
- Vesicular and plasma membrane transporters for neurotransmitters.Cold Spring Harbor perspectives in biology · 2012Review
- Vacuolar H(+)-ATPase subunits Voa1 and Voa2 cooperatively regulate secretory vesicle acidification, transmitter uptake, and storage.Molecular biology of the cell · 2011Article
- Molecular mechanisms of go signaling.Neuro-Signals · 2009Review
- An obligatory requirement for the heterotrimeric G protein Gi3 in the antiautophagic action of insulin in the liver.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Galphao2 regulates vesicular glutamate transporter activity by changing its chloride dependence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Somatostatin inhibits exocytosis in rat pancreatic alpha-cells by G(i2)-dependent activation of calcineurin and depriming of secretory granules.The Journal of physiology · 2001Article
- Synaptic vesicle transporter expression regulates vesicle phenotype and quantal size.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- The neuronal monoamine transporter VMAT2 is regulated by the trimeric GTPase Go(2).The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Posttranslational modification of Galphao1 generates Galphao3, an abundant G protein in brain.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Secretory vesicles store neurotransmitters that are released by exocytosis. Their membrane contains transporters responsible for transmitter loading that are driven by an electrochemical proton gradient across the vesicle membrane. We have now examined whether uptake of noradrenaline is regulated by heterotrimeric G proteins. In streptolysin O-permeabilized PC 12 cells, GTP-analogues and AlF4- inhibited noradrenaline uptake, an effect that was sensitive to treatment with pertussis toxin. Inhibition of uptake was prevented by Galphao-specific antibodies and mimicked by purified activated Galphao2. No effect was seen when Galphao2 in its inactive GDP-bound form or purified activated Galphao1, Galphai1 and Galphai2 were tested. Down-regulation of uptake remained unchanged when exocytosis was inhibited by the light chain of tetanus toxin. Vesicular acidification was not affected whereas binding of [3H]reserpine was reduced by GTPgammaS and Galphao2. These data suggest that the monoamine transporter rather than the vacuolar ATPase is affected. We conclude that catecholamine uptake is controlled by Galphao2, suggesting a novel function for heterotrimeric G proteins in the control of neurotransmitter storage.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.