ArticleThe EMBO journal1998
Three amino acids in the C-linker are major determinants of gating in cyclic nucleotide-gated channels.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 77 citations in OpenAlex.
- Structural basis of properties, mechanisms, and channelopathy of cyclic nucleotide-gated channels.Channels (Austin, Tex.) · 2023Review
- Structure of the human cone photoreceptor cyclic nucleotide-gated channel.Nature structural & molecular biology · 2022Article
- Article
- Oxidative modulation of voltage-gated potassium channels.Antioxidants & redox signaling · 2014Review
- Article
- Inner activation gate in S6 contributes to the state-dependent binding of cAMP in full-length HCN2 channel.The Journal of general physiology · 2012Article
- CNGA3 is expressed in inner ear hair cells and binds to an intracellular C-terminus domain of EMILIN1.The Biochemical journal · 2012Article
- Structural insights into conformational changes of a cyclic nucleotide-binding domain in solution from Mesorhizobium loti K1 channel.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Role of the S4-S5 linker in CNG channel activation.Biophysical journal · 2010Article
- Solution structure of the Mesorhizobium loti K1 channel cyclic nucleotide-binding domain in complex with cAMP.EMBO reports · 2009Article
- Cyclic nucleotide-regulated cation channels.The Journal of biological chemistry · 2009Review
- The structure of the prokaryotic cyclic nucleotide-modulated potassium channel MloK1 at 16 A resolution.Structure (London, England : 1993) · 2007Article
- Function and dysfunction of CNG channels: insights from channelopathies and mouse models.Molecular neurobiology · 2007Review
- The carboxyl-terminal region of cyclic nucleotide-modulated channels is a gating ring, not a permeation path.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Ion channel structure and the promise of bacteria: cyclic nucleotide-gated channels in the queue.The Journal of general physiology · 2004Article
- A cyclic nucleotide modulated prokaryotic K+ channel.The Journal of general physiology · 2004Article
- Molecular basis of an inherited form of incomplete achromatopsia.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
- Disruption of an intersubunit interaction underlies Ca2+-calmodulin modulation of cyclic nucleotide-gated channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Cyclic nucleotide-gated channels: shedding light on the opening of a channel pore.Nature reviews. Neuroscience · 2001Review
- Molecular regions controlling the activity of CNG channels.The Journal of general physiology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The activation of cyclic nucleotide-gated (CNG) channels is a complex process comprising the initial ligand binding and a consecutive allosteric transition from a closed to an open configuration. The cone and olfactory CNG channels differ considerably in cyclic nucleotide affinity and efficacy. In each channel, the cyclic nucleotide-binding site is connected to the last transmembrane segment of the channel by a linker peptide (C-linker) of approximately 90 amino acids. Here we report that replacement of three amino acids in the cone C-linker by the corresponding amino acids of the olfactory channel (I439V, D481A and D494S) profoundly enhanced the cAMP efficacy and increased the affinities for cAMP and cGMP. Unlike the wild-type cone channel, the mutated channel exhibited similar single-channel kinetics for both cGMP and cAMP, explaining the increase in cAMP efficacy. We thus conclude that the identified amino acids are major determinants of channel gating.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.