ArticleThe EMBO journal1998
Interstitial deletions and intrachromosomal amplification initiated from a double-strand break targeted to a mammalian chromosome.
Article in The EMBO journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 117 citations in OpenAlex.
- Meiotic Chromosomal Abnormality Detected in a Heterozygote ofFrontiers in plant science · 2022Article
- Molecular Biology of theCells · 2021Review
- Stem cell-derived clade F AAVs mediate high-efficiency homologous recombination-based genome editing.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- High-resolution mapping of the pericentromeric region on wheat chromosome arm 5AS harbouring the Fusarium head blight resistance QTL Qfhs.ifa-5A.Plant biotechnology journal · 2018Article
- Novel role for non-homologous end joining in the formation of double minutes in methotrexate-resistant colon cancer cells.Journal of medical genetics · 2015Article
- Amplicon rearrangements during the extrachromosomal and intrachromosomal amplification process in a glioma.Nucleic acids research · 2014Article
- Characterization at nucleotide resolution of the homogeneously staining region sites of insertion in two cancer cell lines.Nucleic acids research · 2013Article
- A mechanism of gene amplification driven by small DNA fragments.PLoS genetics · 2012Article
- Different DNA-PKcs functions in the repair of radiation-induced and spontaneous DSBs within interstitial telomeric sequences.Chromosoma · 2011Article
- DNA damage, somatic aneuploidy, and malignant sarcoma susceptibility in muscular dystrophies.PLoS genetics · 2011Article
- Selective regain of egfr gene copies in CD44+/CD24-/low breast cancer cellular model MDA-MB-468.BMC cancer · 2010Article
- Efficient repair of DNA double-strand breaks in malignant cells with structural instability.Mutation research · 2010Article
- Chromosome instability is common in human cleavage-stage embryos.Nature medicine · 2009Article
- Ancient intron insertion sites and palindromic genomic duplication evolutionally shapes an elementally functioning membrane protein family.BMC evolutionary biology · 2007Article
- High-resolution mapping identifies a commonly amplified 11q13.3 region containing multiple genes flanked by segmental duplications.Human genetics · 2007Article
- Genome position and gene amplification.Genome biology · 2007Article
- On the mechanism of gene amplification induced under stress in Escherichia coli.PLoS genetics · 2006Article
- Mutually exclusive promoter hypermethylation patterns of hMLH1 and O6-methylguanine DNA methyltransferase in colorectal cancer.The Journal of molecular diagnostics : JMD · 2006Article
- A mechanism of palindromic gene amplification in Saccharomyces cerevisiae.Genes & development · 2005Article
- Monosomy 1p36 breakpoints indicate repetitive DNA sequence elements may be involved in generating and/or stabilizing some terminal deletions.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interstitial deletions of tumour suppressor genes and amplification of oncogenes are two major manifestations of chromosomal instability in tumour cells. The development of model systems allowing the study of the events triggering these processes is of major clinical importance. Using the properties of the I-SceI nuclease to introduce a localized double-strand break (DSB) in a mammalian chromosome carrying its target sequence, we demonstrate here that both types of mutations can be initiated by non-conservative DSB repair pathways. In our system, I-SceI activity dissociates a transfected gpt gene from its promoter, allowing the isolation of gpt- clones. Our results show that intrachromatid single-strand annealing events occur frequently, giving rise to interstitial deletions not accompanied by other chromosomal rearrangements. We also observed that, when present in the cells, extrachromosomal DNA molecules are integrated preferentially at the broken locus. Taking advantage of the insertion of the I-SceI recognition sequence telomeric to and close to the dihydrofolate reductase gene, we show that a less frequent outcome of I-SceI activity is the initiation of cycles of intrachromosomal amplification of this marker, from breaks at a site merging with the enzyme target.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.