ArticlePlant molecular biology1997
Analysis of microspore-specific promoters in transgenic tobacco.
Article in Plant molecular biology, 1997. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 52 citations in OpenAlex.
- Application of rice microspore-preferred promoters to manipulate early pollen development in Arabidopsis: a heterologous system.Plant reproduction · 2016Article
- Engineered selective plant male sterility through pollen-specific expression of the EcoRI restriction endonuclease.Plant biotechnology journal · 2016Article
- Functional analysis of a rice late pollen-abundant UDP-glucose pyrophosphorylase (OsUgp2) promoter.Molecular biology reports · 2011Article
- Pollen-specific expression of Oryza sativa indica pollen allergen gene (OSIPA) promoter in rice and Arabidopsis transgenic systems.Molecular biotechnology · 2011Article
- The tobacco MAP215/Dis1-family protein TMBP200 is required for the functional organization of microtubule arrays during male germline establishment.Journal of experimental botany · 2010Article
- Article
- Construction and behavior of engineered minichromosomes in maize.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Identification of microspore-active promoters that allow targeted manipulation of gene expression at early stages of microgametogenesis in Arabidopsis.BMC plant biology · 2006Article
- The Oryza sativa no pollen (Osnop) gene plays a role in male gametophyte development and most likely encodes a C2-GRAM domain-containing protein.Plant molecular biology · 2005Article
- Small heat shock proteins are differentially regulated during pollen development and following heat stress in tobacco.Plant molecular biology · 2005Article
- Control of male gametophyte development.The Plant cell · 2004Review
- Selection and orientation of adjacent genes influences DAM-mediated male sterility in transformed maize.Transgenic research · 2001Article
- Isolation and characterization of an anther-specific gene, RA8, from rice (Oryza sativa L.).Plant molecular biology · 1999Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In order to modify the early stages of pollen development in a transgenic context microspore-specific promoters are required. We tested two putatively microspore-specific promoters, the Bp4 promoter from rapeseed and the NTM19 promoter from tobacco. Expression of the gus and barnase reporter genes under the control of these two promoters was studied in transgenic tobacco. Contrary to expectations, the Bp4 promoter became active only after the first pollen mitosis, and not in the microspores. The NTM19 promoter turned out to be highly microspore-specific and directed very high levels of gus expression to the unicellular microspores. The NTM19-barnase transgene caused cell-autonomous death at the mid-unicellular microspore stage, whereas Bp4-barnase induced cell ablation of early to mid-bicellular pollen. Both promoter-barnase transgenes did not affect the sporophyte and were inherited through the female germline. These results show that both the NTM19 and Bp4 promoters are expressed only in the male germline, and that the NTM19 promoter is an excellent tool to direct high levels of transgene expression exclusively to the microspores. This may have important biotechnological applications.
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