ArticleThe Journal of cell biology1998
Detection of dimers of dimers of human leukocyte antigen (HLA)-DR on the surface of living cells by single-particle fluorescence imaging.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 62 citations in OpenAlex.
- Quantitative nanoscale imaging shows peptide-MHC I complexes are monomeric and spatially regulated in human dendritic cells.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing.Nature methods · 2022Article
- Tetraspanin-5-mediated MHC class I clustering is required for optimal CD8 T cell activation.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Crystal structure of a complete ternary complex of T-cell receptor, peptide-MHC, and CD4.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Physical and functional bivalency observed among TCR/CD3 complexes isolated from primary T cells.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Constitutively active Lck kinase in T cells drives antigen receptor signal transduction.Immunity · 2010Article
- Zinc induces dimerization of the class II major histocompatibility complex molecule that leads to cooperative binding to a superantigen.The Journal of biological chemistry · 2007Article
- The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The immune function of MHC class II molecules mutated in the putative superdimer interface.Molecular and cellular biochemistry · 2005Article
- Detecting and quantifying colocalization of cell surface molecules by single particle fluorescence imaging.Biophysical journal · 2003Article
- Vesicle trafficking and cell surface membrane patchiness.Biophysical journal · 2001Article
- Tracking single proteins within cells.Biophysical journal · 2000Article
- The immunological synapse and the actin cytoskeleton: molecular hardware for T cell signaling.Nature immunology · 2000Review
- Cholesterol-dependent clustering of IL-2Ralpha and its colocalization with HLA and CD48 on T lymphoma cells suggest their functional association with lipid rafts.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking.Biophysical journal · 1999Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The technique of single-particle fluorescence imaging was used to investigate the oligomeric state of MHC class II molecules on the surface of living cells. Cells transfected with human leukocyte antigen (HLA)-DR A and B genes were labeled at saturation with a univalent probe consisting of Fab coupled to R-phycoerythrin. Analysis of the intensities of fluorescent spots on the cell surface revealed the presence of single and double particles consistent with the simultaneous presence of HLA-DR heterodimers and dimers of dimers. The proportion of double particles was lower at 37 degrees C than at 22 degrees C, suggesting that the heterodimers and dimers of dimers exist in a temperature-dependent equilibrium. These results are discussed in the context of a possible role for HLA-DR dimers of dimers in T cell receptor-MHC interactions. The technique is validated by demonstrating that fluorescence imaging can distinguish between dimers and tetramers of human erythrocyte spectrin deposited from solution onto a solid substrate. The methodology will have broad applicability to investigation of the oligomeric state of immunological and other membrane-bound receptors in living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.