ArticleThe Journal of cell biology1998
Sphingomyelinase treatment induces ATP-independent endocytosis.
Article in The Journal of cell biology, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
79 citing papers in PubMed, 203 citations in OpenAlex.
- Multi-omics reveal critical roles of phosphatidylcholine and sphingomyelin in antipsychotic efficacy for schizophrenia.Signal transduction and targeted therapy · 2025Article
- The Effect of Ceramide Ratio on the Membrane Curvature of Mimetic Models of Matrix Vesicles.ACS physical chemistry Au · 2025Article
- Adhesion energy controls lipid binding-mediated endocytosis.Nature communications · 2024Article
- Mechano-regulation by clathrin pit-formation and passive cholesterol-dependent tubules during de-adhesion.Cellular and molecular life sciences : CMLS · 2024Article
- Bucket lists must be completed during cell death.Trends in cell biology · 2023Review
- Review
- Severity of COVID-19 Patients Predicted by Serum Sphingolipids Signature.International journal of molecular sciences · 2021Article
- Review
- Anti-flavivirus Properties of Lipid-Lowering Drugs.Frontiers in physiology · 2021Review
- The Manifold Roles of Sphingolipids in Viral Infections.Frontiers in physiology · 2021Review
- The role of lipid species in membranes and cancer-related changes.Cancer metastasis reviews · 2020Review
- Tumor-derived exosomes (TDEs): How to avoid the sting in the tail.Medicinal research reviews · 2020Review
- On the existence of endocytosis driven by membrane phase separations.Biochimica et biophysica acta. Biomembranes · 2020Review
- Exosome Biogenesis in the Protozoa ParasiteCells · 2019Article
- Solving the secretory acid sphingomyelinase puzzle: Insights from lysosome-mediated parasite invasion and plasma membrane repair.Cellular microbiology · 2019Review
- Approaches for probing and evaluating mammalian sphingolipid metabolism.Analytical biochemistry · 2019Review
- Role of Sphingomyelin in Alphaherpesvirus Entry.Journal of virology · 2019Article
- Interrelations of Sphingolipid and Lysophosphatidate Signaling with Immune System in Ovarian Cancer.Computational and structural biotechnology journal · 2019Article
- Anandamide Revisited: How Cholesterol and Ceramides Control Receptor-Dependent and Receptor-Independent Signal Transmission Pathways of a Lipid Neurotransmitter.Biomolecules · 2018Review
- Lipid signaling to membrane proteins: From second messengers to membrane domains and adapter-free endocytosis.The Journal of general physiology · 2018Review
19 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
ATP hydrolysis has been regarded as a general requirement for internalization processes in mammalian cells. We found, however, that treatment of ATP-depleted macrophages and fibroblasts with exogenous sphingomyelinase (SMase) rapidly induces formation of numerous vesicles that pinch off from the plasma membrane; the process is complete within 10 min after adding SMase. By electron microscopy, the SMase-induced vesicles are approximately 400 nm in diameter and lack discernible coats. 15-30% of plasma membrane is internalized by SMase treatment, and there is no detectable enrichment of either clathrin or caveolin in these vesicles. When ATP is restored to the cells, the SMase-induced vesicles are able to deliver fluid-phase markers to late endosomes/lysosomes and return recycling receptors, such as transferrin receptors, back to the plasma membrane. We speculate that hydrolysis of sphingomyelin on the plasma membrane causes inward curvature and subsequent fusion to form sealed vesicles. Many cell types express a SMase that can be secreted or delivered to endosomes and lysosomes. The hydrolysis of sphingomyelin by these enzymes is activated by several signaling pathways, and this may lead to formation of vesicles by the process described here.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.