ArticleThe Biochemical journal1998
Molecular cloning of rat mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase and detection of the corresponding mRNA and of those encoding the remaining enzymes comprising the ketogenic 3-hydroxy-3-methylglutaryl-CoA cycle in central nervous system of suckling rat.
Article in The Biochemical journal, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Dysregulated lipid metabolism and hypomyelination in postnatal peroxisome-deficientFrontiers in molecular neuroscience · 2026Article
- Neural Metabolic Networks: Key Elements of Healthy Brain Function.Journal of neurochemistry · 2025Review
- Mitochondrial Metabolism in Astrocytes Regulates Brain Bioenergetics, Neurotransmission and Redox Balance.Frontiers in neuroscience · 2020Review
- The specific molecular architecture of plant 3-hydroxy-3-methylglutaryl-CoA lyase.The Journal of biological chemistry · 2019Article
- HMGCS2 is a key ketogenic enzyme potentially involved in type 1 diabetes with high cardiovascular risk.Scientific reports · 2017Article
- Streptozotocin diabetes increases mRNA expression of ketogenic enzymes in the rat heart.Biochimica et biophysica acta. General subjects · 2017Article
- Non-Neuronal Cells in the Hypothalamic Adaptation to Metabolic Signals.Frontiers in endocrinology · 2017Review
- Pyruvate kinase expression (PKM1 and PKM2) in cancer-associated fibroblasts drives stromal nutrient production and tumor growth.Cancer biology & therapy · 2011Article
- Activity-regulated cytoskeleton-associated protein in rodent brain is down-regulated by high fat diet in vivo and by 27-hydroxycholesterol in vitro.Brain pathology (Zurich, Switzerland) · 2009Article
- Metabolic and regulatory roles of leucine in neural cells.Neurochemical research · 2008Review
- Neonatal hypoglycaemia in Nepal 2. Availability of alternative fuels.Archives of disease in childhood. Fetal and neonatal edition · 2000Article
- Structure, function and regulation of pyruvate carboxylase.The Biochemical journal · 1999Review
- Mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase: a control enzyme in ketogenesis.The Biochemical journal · 1999Review
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Authors and funding
6 authors.
Funding
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Abstract
We have investigated, by RNase protection assays in rat brain regions and primary cortical astrocyte cultures, the presence of the mRNA species encoding the three mitochondrially located enzymes acetoacetyl-CoA thiolase, mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase (mt. HMG-CoA synthase) and HMG-CoA lyase (HMG-CoA lyase) that together constitute the ketogenic HMG-CoA cycle. As a prerequisite we obtained a full-length cDNA encoding rat HMG-CoA lyase by degenerate oligonucleotide-primed PCR coupled to a modification of PCR-rapid amplification of cDNA ends (PCR-RACE). We report here: (1) the nucleotide sequence of rat mt. HMG-CoA lyase, (2) detection of the mRNA species encoding all three HMG-CoA cycle enzymes in all regions of rat brain during suckling, (3) approximately twice the abundance of mt. HMG-CoA synthase mRNA in cerebellum than in cortex in 11-day-old suckling rat pups, (4) significantly lower abundances of mt. HMG-CoA synthase mRNA in brain regions derived from rats weaned to a high-carbohydrate/low-fat diet compared with the corresponding regions derived from the suckling rat, and (5) the presence of mt. HMG-CoA synthase mRNA in primary cultures of neonatal cortical astrocytes at an abundance similar to that found in liver of weaned animals. These results provide preliminary evidence that certain neural cell types possess ketogenic potential and might thus have a direct role in the provision of fatty acid-derived ketone bodies during the suckling period.
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