ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1998
Intracellular calcium regulates agrin-induced acetylcholine receptor clustering.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 74 citations in OpenAlex.
- A link between agrin signalling and CaScientific reports · 2022Article
- Cross-GWAS coherence test at the gene and pathway level.PLoS computational biology · 2022Article
- Achieving Acetylcholine Receptor Clustering in Tissue-Engineered Skeletal Muscle ConstructsFrontiers in pharmacology · 2016Article
- Biglycan is an extracellular MuSK binding protein important for synapse stability.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Skeletal muscle IP3R1 receptors amplify physiological and pathological synaptic calcium signals.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Calcium/calmodulin kinase II-dependent acetylcholine receptor cycling at the mammalian neuromuscular junction in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- To build a synapse: signaling pathways in neuromuscular junction assembly.Development (Cambridge, England) · 2010Review
- Article
- Muscle-wide secretion of a miniaturized form of neural agrin rescues focal neuromuscular innervation in agrin mutant mice.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- MuSK signaling at the neuromuscular junction.Journal of molecular neuroscience : MN · 2006Article
- Agrin promotes synaptic differentiation by counteracting an inhibitory effect of neurotransmitter.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- IP3 receptors and associated Ca2+ signals localize to satellite cells and to components of the neuromuscular junction in skeletal muscle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- A role for the juxtamembrane domain of beta-dystroglycan in agrin-induced acetylcholine receptor clustering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Clustering of nicotinic acetylcholine receptors: from the neuromuscular junction to interneuronal synapses.Molecular neurobiology · 2002Review
- Effects of purified recombinant neural and muscle agrin on skeletal muscle fibers in vivo.The Journal of cell biology · 2001Article
- Src, Fyn, and Yes are not required for neuromuscular synapse formation but are necessary for stabilization of agrin-induced clusters of acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Evidence of an agrin receptor in cortical neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Roles of rapsyn and agrin in interaction of postsynaptic proteins with acetylcholine receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Neurotrophins regulate agrin-induced postsynaptic differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Specific agrin isoforms induce cAMP response element binding protein phosphorylation in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Agrin is an extracellular matrix protein that directs neuromuscular junction formation. Early signal transduction events in agrin-mediated postsynaptic differentiation include activation of a receptor tyrosine kinase and phosphorylation of acetylcholine receptors (AChRs), but later steps in this pathway are unknown. Here, we have investigated the role of intracellular calcium in agrin-induced AChR clustering on cultured myotubes. Clamping intracellular calcium levels by loading with the fast chelator BAPTA inhibited agrin-induced AChR aggregation. In addition, preexisting AChR aggregates dispersed under these conditions, indicating that the maintenance of AChR clusters is similarly dependent on intracellular calcium fluxes. The decrease in AChR clusters in BAPTA-loaded cells was dose-dependent and reversible, and no change in the number or mobility of AChRs was observed. Clamping intracellular calcium did not block agrin-induced tyrosine phosphorylation of the AChR beta-subunit, indicating that intracellular calcium fluxes are likely to act downstream from or parallel to AChR phosphorylation. Finally, the targets of the intracellular calcium are likely to be close to the calcium source, since agrin-induced AChR clustering was unaffected in cells loaded with EGTA, a slower-binding calcium chelator. These findings distinguish a novel step in the signal transduction mechanism of agrin and raise the possibility that the pathways mediating agrin- and activity-driven changes in synaptic architecture could intersect at the level of intracellular calcium fluxes.
Indexed as
Identifiers
9425009PMC6792545W2108147568What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.