ArticleInfection and immunity1998
Identification and characterization of protective T cells in hsp65 DNA-vaccinated and Mycobacterium tuberculosis-infected mice.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
50 citing papers in PubMed, 141 citations in OpenAlex.
- Secreted proteins ofFrontiers in tuberculosis · 2026Review
- Advances in the development of new vaccines for tuberculosis and Brazil's role in the effort forward the end TB strategy.Memorias do Instituto Oswaldo Cruz · 2024Article
- Mycobacterial Hsp65 antigen upregulates the cellular immune response of healthy individuals compared with tuberculosis patients.Human vaccines & immunotherapeutics · 2017Article
- B cells expressing IL-10 mRNA modulate memory T cells after DNA-Hsp65 immunization.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2015Article
- Genomic expression catalogue of a global collection of BCG vaccine strains show evidence for highly diverged metabolic and cell-wall adaptations.Scientific reports · 2015Article
- Complexity and Controversies over the Cytokine Profiles of T Helper Cell Subpopulations in Tuberculosis.Journal of immunology research · 2015Review
- High-frequency vaccine-induced CD8⁺ T cells specific for an epitope naturally processed during infection with Mycobacterium tuberculosis do not confer protection.European journal of immunology · 2014Article
- Mycobacterium leprae Hsp65 administration reduces the lifespan of aged high antibody producer mice.Immunity & ageing : I & A · 2014Article
- Immunotherapy of tuberculosis with Mycobacterium leprae Hsp65 as a DNA vaccine triggers cross-reactive antibodies against mammalian Hsp60 but not pathological autoimmunity.Human vaccines & immunotherapeutics · 2014Article
- BCG and BCG/DNAhsp65 vaccinations promote protective effects without deleterious consequences for experimental autoimmune encephalomyelitis.Clinical & developmental immunology · 2013Article
- Antigen-presenting cells transfected with Hsp65 messenger RNA fail to treat experimental tuberculosis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2012Article
- Protection conferred by heterologous vaccination against tuberculosis is dependent on the ratio of CD4(+) /CD4(+) Foxp3(+) cells.Immunology · 2012Article
- Immunotherapeutic role of Ag85B as an adjunct to antituberculous chemotherapy.Journal of immune based therapies and vaccines · 2011Article
- B cells Can Modulate the CD8 Memory T Cell after DNA Vaccination Against Experimental Tuberculosis.Genetic vaccines and therapy · 2011Article
- Intranasal vaccination with messenger RNA as a new approach in gene therapy: use against tuberculosis.BMC biotechnology · 2010Article
- Helminth coinfection does not affect therapeutic effect of a DNA vaccine in mice harboring tuberculosis.PLoS neglected tropical diseases · 2010Article
- DNA vaccine containing the mycobacterial hsp65 gene prevented insulitis in MLD-STZ diabetes.Journal of immune based therapies and vaccines · 2009Article
- Evasion and subversion of antigen presentation by Mycobacterium tuberculosis.Tissue antigens · 2009Review
- Immune response to vaccination with DNA-Hsp65 in a phase I clinical trial with head and neck cancer patients.Cancer gene therapy · 2009Article
- Protection against tuberculosis by a single intranasal administration of DNA-hsp65 vaccine complexed with cationic liposomes.BMC immunology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunization by intramuscular injection of plasmid DNA expressing mycobacterial 65-kDa heat shock protein (hsp65) protects mice against challenge with virulent Mycobacterium tuberculosis H37Rv. During infection or after immunization, CD4+/CD8- and CD8+/CD4- hsp65-reactive T cells increased equally in spleens. During infection, the majority of these cells were weakly CD44 positive (CD44(lo)) and produced interleukin 4 (IL-4) whereas after immunization the majority were highly CD44 positive (CD44(hi)) and produced gamma interferon (IFN-gamma). In adoptive transfer of protection to naive mice, the total CD8+/CD4- cell population purified from spleens of immunized mice was more protective than that from infected mice. When the cells were separated into CD4+/CD8- and CD8+/CD4- types and then into CD44(hi) and CD44(lo) types, CD44(lo) cells were essentially unable to transfer protection, the most protective CD44(hi) cells were CD8+/CD4-, and those from immunized mice were much more protective than those from infected mice. Thus, whereas the CD44(lo) IL-4-producing phenotype prevailed during infection, protection was associated with the CD8+/CD44(hi) IFN-gamma-producing phenotype that predominated after immunization. This conclusion was confirmed and extended by analysis of 16 hsp65-reactive T-cell clones from infected mice and 16 from immunized mice; the most protective clones, in addition, displayed antigen-specific cytotoxicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.