ArticleInfection and immunity1998
Identification of a genetic locus essential for serotype b-specific antigen synthesis in Actinobacillus actinomycetemcomitans.
Article in Infection and immunity, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 49 citations in OpenAlex.
- The Trimeric Autotransporter Adhesin EmaA and Infective Endocarditis.Pathogens (Basel, Switzerland) · 2024Review
- Genomic Islands Shape the Genetic Background of Both JP2 and Non-JP2Pathogens (Basel, Switzerland) · 2022Article
- The serotype a-EmaA adhesin ofMicrobiology (Reading, England) · 2022Article
- O-Polysaccharide Plays a Major Role on the Virulence and Immunostimulatory Potential ofFrontiers in immunology · 2020Article
- A network-based approach to identify substrate classes of bacterial glycosyltransferases.BMC genomics · 2014Article
- Genomic Stability of Aggregatibacter actinomycetemcomitans during Persistent Oral Infection in Human.PloS one · 2013Article
- ABC transporters involved in export of cell surface glycoconjugates.Microbiology and molecular biology reviews : MMBR · 2010Review
- Glycosylation of the collagen adhesin EmaA of Aggregatibacter actinomycetemcomitans is dependent upon the lipopolysaccharide biosynthetic pathway.Journal of bacteriology · 2010Article
- Francisella tularensis T-cell antigen identification using humanized HLA-DR4 transgenic mice.Clinical and vaccine immunology : CVI · 2010Article
- Transcriptional and translational analysis of biofilm determinants of Aggregatibacter actinomycetemcomitans in response to environmental perturbation.Infection and immunity · 2009Article
- The dTDP-4-dehydro-6-deoxyglucose reductase encoding fcd gene is part of the surface layer glycoprotein glycosylation gene cluster of Geobacillus tepidamans GS5-97T.Glycobiology · 2007Article
- Surface polysaccharides and iron-uptake systems of Actinobacillus pleuropneumoniae.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2004Review
- Actinobacillus actinomycetemcomitans serotype d-specific antigen contains the O antigen of lipopolysaccharide.Infection and immunity · 2003Article
- Actinobacillus actinomycetemcomitans serotype b lipopolysaccharide mediates coaggregation with Fusobacterium nucleatum.Infection and immunity · 2003Article
- Novel Aeromonas hydrophila PPD134/91 genes involved in O-antigen and capsule biosynthesis.Infection and immunity · 2002Article
- Structural and genetic analyses of O polysaccharide from Actinobacillus actinomycetemcomitans serotype f.Infection and immunity · 2001Article
- Identification of Actinobacillus actinomycetemcomitans serotypes by multiplex PCR.Journal of clinical microbiology · 2001Article
- Nonspecific adherence by Actinobacillus actinomycetemcomitans requires genes widespread in bacteria and archaea.Journal of bacteriology · 2000Article
- A cluster of genes involved in polysaccharide biosynthesis from Enterococcus faecalis OG1RF.Infection and immunity · 1998Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large gene cluster associated with the biosynthesis of the serotype-specific polysaccharide antigen (SPA) of Actinobacillus actinomycetemcomitans Y4 (serotype b) was cloned and characterized. Western blot analysis showed that Escherichia coli DH5alpha, containing a plasmid carrying this cluster, produced a polysaccharide which reacted with a monoclonal antibody directed against the SPA of A. actinomycetemcomitans Y4. High-performance liquid chromatography analysis indicated that the polysaccharide produced by an E. coli transformant, as well as A. actinomycetemcomitans Y4 SPA, was composed of rhamnose and fucose. Furthermore, using various derivatives of the plasmid, we demonstrated that the cloned 13-kb BssHII-BspHI fragment was indispensable for SPA synthesis in E. coli DH5alpha. The 24,909-bp nucleotide sequence, which included this fragment and its flanking regions, was determined. In the sequenced area, 24 open reading frames (ORFs) with the same orientation were found. Most of these were located sequentially within a short distance of each other. Many of the deduced amino acid sequences were similar to the gene products of the polysaccharide synthetic genes of other bacteria. The average G+C content (37.7%) of all 24 ORFs in the sequenced area was lower than that (45.6%) of the whole chromosome of A. actinomycetemcomitans Y4. It is noteworthy the average G+C content of the nine ORFs in the 8.5-kb central region of the 13-kb BssHII-BspHI fragment indispensable for SPA synthesis in E. coli was found to be especially low (27.0%).
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